家蚕微孢子图像自动聚焦系统的设计与实现  

Design and Implementation of Efficient Microsporidia Image Auto-focus System

作　　者：吴恢[1] 杨光友[2] 胡兴明[1] 马志艳[2] 范锦[1] 邓文[1] 叶楚华[1] 

机构地区：[1]湖北省农业科学院经济作物研究所,武汉430064 [2]湖北工业大学,武汉430064

出　　处：《湖北农业科学》2014年第24期6120-6123,共4页Hubei Agricultural Sciences

基　　金：湖北省自然科学基金重点项目(2010CBB0081)

摘　　要：针对家蚕微孢子人工检测的传统作业方式,设计并实现了一种以PC机为控制核心的微孢子显微成像自动聚焦系统。以普通光学显微镜为基础,对载物台三维运动平台进行了高精度的设计改造;优化了针对微孢子显微图像的清晰度评价函数,为平衡调焦范围和调焦速度的矛盾,设计了"二步法"调焦策略。结果表明,该系统自动聚焦的准确度和实时性基本上满足使用需要。Based on the traditional nosema bombycis artificial microscopy, a novel optical auto-focus microscopy system con- trolled by PC was designed and implemented. Firstly, based on an ordinary optical microscope, a high-precision three-di- mensional movement stage was designed. Secondly, an appropriate spore microscopic image definition evaluation function was preferred. Taking the focusing range and focusing speed into account, the system used a ＂two-step＂ focus strategy. Results showed that the proposed scheme satified the requirements of auto-focusing with accuracy and speed.

关 键 词：微孢子检测 自动调焦 聚焦函数 显微镜 

分 类 号：TP391.4[自动化与计算机技术—计算机应用技术]