水稻Waxy基因型鉴定及其对稻米品质性状的影响  

Genotype Identification of Waxy Rice and Its Effects on Grain Quality Traits

作　　者：蔡海亚[1] 徐得泽[1] 周雷[1] 闸雯俊[1] 李三和[1] 刘凯[1] 杨国才[1] 焦春海[1] 游艾青[1] 

机构地区：[1]湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2014年第24期6131-6133,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金青年基金项目(31301288);国家"973"计划项目(2013CBA01405);杂交水稻国家重点实验室(武汉大学)开放项目(KF201402);粮食作物种质创新与遗传改良湖北省重点实验室开放项目(2014lzjj01);科技部国际合作专项(2011DFB31620)

摘　　要：水稻(Oryza sativa L.)Waxy基因第一内含子+1位G→T突变导致直链淀粉含量降低,针对该位点开发了鉴别两种不同Waxy基因型的标记,通过对143份水稻微核心种质材料进行检测,结果表明,该基因同时影响水稻直链淀粉含量和胶稠度,其中G型等位基因直链淀粉含量平均值为22.53%,极显著高于T型的8.89%(P<0.01),而G型材料胶稠度为52.11 mm,极显著低于T型(88.33 mm)(P<0.01),并且直链淀粉含量和胶稠度在所有供试材料中表现出极显著负相关(相关系数-0.816,P<0.01),因此,Waxy基因同时影响水稻直链淀粉含量与胶稠度两个品质相关性状,在改良稻米品质的分子育种中将具有重要的应用价值。The base substitution （G→T） at ＋1 loci of the first intron of Waxy gene resulted in the decrease of AC. Identification marker targeted at this SNP loci site of these two different Waxy allele were developed . Results of 143 rice mini-core collections showed that Waxy gene affected amylose content （AC） and gel consistency （GC） simultaneously. The average AC was 22.53% for G-type allele which was significantly higher than that of T-type allele （8.89%）. Its GC was significantly lower than that of T-type allele with （52.11 mm and 88.33 mm, respectively）. AC was significantly negatively correlated with GC （correlation=-0.816, P〈0.01）. Waxy gene affected grain quality traits AC and GC, having significant application in rice molecular breeding.

关 键 词：水稻(Oryza SATIVA L.) 直链淀粉含量 胶稠度 

分 类 号：S511[农业科学—作物学]