高+G_z应激致脑损伤的分子机制与低+G_z预适应的保护作用  

Study on the molecular mechanism of high +G_z induced brain injury and the protection of low +G_Z preconditioning

作　　者：吴峰[1] 陈良恩[1] 赵安东[1] 葛华[1] 李侠[1] 詹皓[1] 

机构地区：[1]空军航空医学研究所,北京100142

出　　处：《空军医学杂志》2014年第4期185-188,共4页Medical Journal of Air Force

基　　金：全军后勤科研计划课题(CWS11J194)

摘　　要：目的筛选与高+G_z暴露和低+G_z预适应相关的差异表达基因,并进行生物信息学分析,加深对加速度应激致脑损伤分子机制的认识。方法将大鼠分为对照组、+10 G_z应激组和低+G_z预适应组,在+10 G_z暴露后24 h分别提取大鼠海马组织总RNA,利用基因芯片技术进行差异基因筛选,基于京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)数据库进行Pathway分析。结果与对照组比较,+10 G_z应激组筛选出差异基因846个涉及56个KEGG信号通路;低+G_z预适应组筛选出差异基因992个,涉及49个KEGG信号通路。结论对高+G_z应激和低+G_z预适应组的差异基因表达的分析发现,差异基因表达涉及多条信号通路,可为深入研究高+G_z应激致脑损伤的分子机制和探讨有关防护措施的效果提供实验依据。ObjectiveTo identify differentially expressed genes in hippocampus of rats with high ＋Gz stress exposure and low ＋Gz preconditioning,so as to further understand the molecular mechanisms of positive acceleration induced brain injury. MethodsMale SD rats were randomly assigned to three groups： control group, ＋10 Gz stress group and low ＋Gz preconditioning group. Total RNA was prepared from experimental rats hippocampus after 24 hour of ＋10 Gz exposure. The method of microarray was applied to detect the differentially expressed genes and analyze the pathways through KEGG database.ResultsThe results showed that there were 846 genes differentially expressed in ＋10 Gz stress group and 992 genes in low ＋Gz preconditioning group compared with the control group. There were 56 KEGG pathways in ＋10 Gz stress group, 49 KEGG pathways in low ＋Gz preconditioning group.ConclusionsThe results indicated that the differentially expressed genes were involved in different signaling pathways. These patterns of gene expression might contribute to understanding the molecular mechanism of high ＋Gz stress-induced brain injury and its countermeasures.

关 键 词：加速度应激 基因芯片 海马 大鼠 

分 类 号：R85[医药卫生—航空、航天与航海医学]