3′-大豆苷元磺酸钠对去血清所致海马神经元损伤的保护作用  

Protective Effect of 3′-daidzein Suffocates Sodium on Serum Deprivation-induced Hippocampal Neuronal Cell Damage

作　　者：曾靖[1] 薛进华[1] 黎晓[1] 黄志华[1] 

机构地区：[1]赣南医学院,江西省脑血管药理重点实验室

出　　处：《神经药理学报》2013年第3期15-18,共4页Acta Neuropharmacologica

基　　金：国家自然科学基金资助项目(No.81160399);江西省高等学校科技落地计划项目(No.KJLD13085)

摘　　要：目的：探讨3’-大豆苷元磺酸钠（3’daidzein suffocatessodium，3’-DSS）对去血清所致海马神经元损伤的保护作用。方法：取新生SD大鼠（0～24h）海马区制成单细胞悬液进行体外培养，待细胞发育成熟后，用去血清的培养基制作海马神经元损伤模型，造模同时给予3’-大豆苷元磺酸钠（3．0、10．0、30．0μmol·L^-1）处理，继续培养24h，采用MTT法检测细胞活性；并观察细胞形态的改变；取培养上清液，测定去血清海马神经元中乳酸脱氢酶（1actate dehydrogenase，LDH）活性。结果：形态学观察模型组海马神经元细胞膜边界相对模糊，细胞皱缩，神经突触回缩，少量细胞贴壁不牢；其上清液中去血清海马神经元中LDH活性升高；MTT法检测结果显示去血清海马神经元活性降低。给予3’-大豆苷元磺酸钠处理后，镜下观察可见细胞膜边界清楚，胞体饱满，神经突触回缩不明显，细胞贴壁良好；去血清海马神经元细胞活性升高，去血清海马神经元LDH活性降低。结论：3’-大豆苷元磺酸钠对去血清所致海马神经元损伤具有显著的保护作用。Objective：To study the protective effect of 3＇-daidzein sulfonate sodium （3＇- DSS） on hippocampal neuronal cell damage induced by serum deprivation. Methods：Monolayer cell suspension of newborn SD rats （0-24 h）hippocampus was cultured in vitro. As the cells matured, hippocampal neuronal cell injury model was made by using serum-free medium. After treated with 3＇-DS S （3.0, 10.0,30.0 μmol·L^-1）and cultivate for 24 h, the cell viability was detected by MTT assay,the cell shape changes were observed,the lactate dehydrogenase （LDH） activity of serum-removed hippocampal neurons was detected. Results：In the 3＇-DSS treating groups, comparing with the control group, the boundary of hippocampal neuron cell membrane is clearer, cell bodies are normal, the retraction of synapses is relieved and the cell adherence to the wall normal. The cell viability is increased in treated groups, while the LDH activity of serum- removed hippocampal neurons is decreased. The result of MTT revealed cell viability of serumremoved hippocampal neurons was increased in 3＇-DSS treating group as compared to the control group. Conclusion：3＇-DSS shows significant protective effect on hippocampal neuronal cell damage induced by removing serum.

关 键 词：3’-大豆苷元磺酸钠 海马神经元 保护作用 

分 类 号：R965[医药卫生—药理学]