氧化苦参碱对视网膜母细胞瘤细胞凋亡的诱导作用及机制  

Oxymatrine induce apoptosis in retinoblastoma cells and its mechanism

作　　者：邱海江[1] 袁穗蓉 张柳[2] 

机构地区：[1]广州市第一人民医院南沙医院,广州511457 [2]广州市第一人民医院,广州510180

出　　处：《广州医药》2015年第1期52-54,共3页Guangzhou Medical Journal

基　　金：2012年广东省建设中医药强省科研课题(20122013)

摘　　要：目的氧化苦参碱对视网膜母细胞瘤细胞SM-106凋亡的诱导作用及机制。方法以不同作用时间(24 h、48 h、72 h)和不同作用浓度(12.5μl/m L、25μl/m L、50μl/m L、100μl/m L)氧化苦参碱处理视网膜母细胞瘤细胞SM-106,分别采用流式细胞仪及western blot检测视网膜母细胞瘤细胞SM-106细胞凋亡及其凋亡因子(Bax、Bcl-2)蛋白表达。结果氧化苦参碱可促进SM-106细胞体外凋亡,上调Bax蛋白表达及Bax/Bcl-2蛋白表达比值,下调Bcl-2蛋白表达,并呈现剂量及时间依赖性。结论氧化苦参碱可诱导视网膜母细胞瘤细胞SM-106凋亡,调控凋亡因子Bax、Bcl-2的表达是其可能作用机制。Objective To evaluate the apoptosis and its mechanism of retinoblastoma cells SM-106 induced by oxymatrine. Methods Retinoblastoma cells SM-106 were treated with different time （24 h, 48 h, 72 h） and different concentrations （ 12. 5 μl/mL, 25 μl/mL, 50 μl/mL or 100 μ/mL） of oxymatrine. The apoptosis and protein expression of apoptosis factors （Bax and Bcl-2） were respectively determined by flow cytometry and western blot. Results Oxymatrine significantly promoted the SM-106 cells apoptosis in vitro, raised Bax protein expression and Bax/Bcl-2 protein expression ratio, reduced the Bcl-2 protein expression, and showed the dose and time dependent. Conclusion Oxymatrine is able to induce the apoptosis in retinoblastoma cells SM-106. Regulating apoptosis related gene Bax and Bcl-2 expression may be the mechanism of apoptosis.

关 键 词：氧化苦参碱 视网膜母细胞瘤 凋亡 BAX BCL-2 

分 类 号：R285[医药卫生—中药学]