人参三七川芎提取物抑制p53基因表达促进衰老内皮细胞增殖的研究  

Effects of extracts from ginseng, notoginseng and chuanxiong on the proliferation of vascular endothelial cells to delay senescence via the inhibition of p53 gene expression

作　　者：王铭[1] 雷燕[2] 陈连凤 苗立夫 

机构地区：[1]重庆市中医院心内科,重庆400021 [2]中国中医科学院医学实验中心,北京100700 [3]中国医学科学院北京协和医院心内科实验室,北京100730 [4]清华大学第一附属医院心脏中心,北京100016

出　　处：《中华中医药杂志》2015年第2期519-523,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2007CB507406);国家自然科学基金资助项目(No.30973828);中国中医科学院自主选题资助项目(No.Z02151)~~

摘　　要：目的:探讨益气活血中药对血管内皮细胞衰老和p53基因表达的干预作用。方法:分离人脐静脉内皮细胞(HUVEC),传代培养至第4代,血管紧张素Ⅱ(AngⅡ,10-6mol/L,48h)诱导其衰老;分为空白对照组、AngⅡ组、中药提取物组和替米沙坦组;观察各组不同时间点β-半乳糖苷酶染色、CCK-8细胞计数、细胞周期分析、p53 m RNA及蛋白表达。结果:AngⅡ组较空白对照组β-半乳糖苷酶阳性染色细胞数增加(P<0.05);CCK-8检测活细胞数减少(P<0.05);细胞大量停滞于G0/G1期,而S期和G2/M期逐渐消失(P<0.05)。中药提取物组和替米沙坦组β-半乳糖苷酶阳性染色细胞数下降(P<0.05),CCK-8活细胞数增加(P<0.05),细胞周期阻滞改善(P<0.05)。中药早期作用更加明显(P<0.05)。AngⅡ呈时间依赖性上调p53 m RNA和蛋白表达;中药提取物和替米沙坦两组p53表达均明显下调(P<0.05);中药早期作用更加明显(P<0.05)。结论:益气活血中药能够有效地延缓血管内皮细胞衰老,其机制可能与抑制p53基因表达有关。Objective： To explore the intervention effect of extracts from ginseng, notoginseng and chuanxiong on endothelial cells senescence and p53 gene expression. Methods： Human umbilical vein endothelial cells（HUVEC） were obtained and cultured in vitro until fourth passage. HUVECs were intervened by AngⅡ（stimulated with AngⅡ 10-6mol/L for 48h） and divided into control group, AngⅡ group, extracts group and Telmisartan group. β-gal staining, CCK-8, cell cycle analysis and p53 m RNA and protein expression were observed at different time points after intervention. Results： Compared with the control group, the positive cell number of β-gal staining was significantly increased in AngⅡ group（P〈0.05）, the number of CCK-8 surviving cells was greatly reduced in AngⅡ group（P〈0.05）. The cell cycle was arrested at G0/G1 phase, S phase and G2/M phase were disappearing at the same time（P〈0.05）. Extracts and Telmisartan could reduce the positive cell number of β-gal staining and increase the CCK-8 surviving cells and improve the cell cycle arrest（P〈0.05）. The effect of extracts was more prominent in early stage than that of Telmisartan（P〈0.05）. AngⅡ could up-regulate the p53 m RNA and protein expression following time-dependent（P〈0.05）, the extracts and Telmisartan could down-regulate p53 expression simultaneously（P〈0.05）, and the effect of extracts was more prominent in early stage（P〈0.05）. Conclusion： Extracts could delay senescence of vascular endothelial cells via downregulation of p53 expression, the mechanism may be related to the inhibition of p53 expression.

关 键 词：人参三七川芎提取物 血管内皮细胞衰老 P53 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]