毛细管气相色谱法同时测定暑热宁口服液中6个有效成分的含量  

Simultaneous determination of six active ingredient content in Shurening Oral Liquid by capillary pillar gas chromatography method

作　　者：李凤丽[1,2] 李进[1] 高文远[3] 陈涛[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津中医药大学,天津300193 [3]天津大学,天津300193

出　　处：《中华中医药杂志》2015年第2期572-574,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家"重大新药创制"科技重大专项项目(No.2010ZX09102-201)~~

摘　　要：目的:建立同时测定暑热宁口服液中麝香草酚、香荆芥酚、百秋李醇、广藿香酮、厚朴酚、和厚朴酚6个有效成分含量的气相色谱方法。方法:采用气相色谱法,色谱柱为HP-5毛细管色谱柱(30m×0.32mm,0.25μm),进样口温度220℃,检测器温度220℃,程序升温,起始温度110℃,保持10min,以25℃程序升温至230℃,保持9.2min,分流比10∶1,进样量1μL。结果:麝香草酚、香荆芥酚、百秋李醇、广藿香酮、厚朴酚、和厚朴酚进样浓度分别在0.226-2.260mg/m L(r=0.99961),0.1055-1.0550mg/m L(r=0.99954),0.212-2.120mg/m L(r=0.99963),0.114-1.140mg/m L(r=0.99955),0.0828-0.8280mg/m L(r=0.99968),0.072-0.720mg/m L(r=0.99975)成良好线性关系,平均加样回收率(n=9)为100.9%-104.5%(RSD2.4%),99.2%-103.4%(RSD1.9%),97.2%-100.8%(RSD1.9%),96.2%-99.4%(RSD1.7%),96.2%-98.7%(RSD2.1%),95.2%-98.2%(RSD1.8%)。结论:该分析方法简单,可控性强,结果准确,可同时分析暑热宁口服液中的多种主要成分。Objective： To establish the capillary pillar gas chromatography method to simultaneous determine six active ingredient content（thymol, carvacrol, patchouli lee alcohol, pogostone, magnolol and honokiol） in Shurening Oral Liquid. Methods： Using the gas chromatography method, the column was HP-5 capillary column（30m×0.32 mm, 0.25μm）, inlet temperature at 220℃, detector temperature at 220℃, temperature program, initial temperature at 110℃, keeping 10 min, 25℃ temperature programmed to 230℃, keeping 9.2min, split ratio was 10 ： 1, injection volume was 1μL. Results： Thymol, carvacrol, patchouli lee alcohol, pogostone, magnolol and honokiol injection concentration was respectively 0.226-2.260mg/m L（r=0.99961）, 0.1055-1.0550mg/m L（r=0.99954）, 0.212-2.120 mg/m L（r=0.99963）, 0.114-1.140mg/m L（r=0.99955）, 0.0828-0.8280mg/m L（r=0.99968）, 0.072-0.720mg/m L（r=0.99975） as a good linear relationship. The average recoveries（n=9） was 100.9%-104.5%（RSD2.4%）, 99.2%-103.4%（RSD1.9%）, 97.2%-100.8%（RSD1.9%）, 96.2%-99.4%（RSD1.7%）, 96.2%-98.7%（RSD2.1%）, 95.2%-98.2%（RSD1.8%）. Conclusion： The analysis method was simple, controllable and accurate, which could simultaneous analyze the main components in a variety of Shurening Oral Liquid.

关 键 词：毛细管气相色谱 暑热宁口服液 麝香草酚 香荆芥酚 百秋李醇 广藿香酮 厚朴酚 和厚朴酚 

分 类 号：R286.0[医药卫生—中药学] O657.71[医药卫生—中医学]