加味千金苇茎汤对慢性阻塞性肺疾病大鼠肺组织SIGIRR蛋白及其mRNA表达的影响  

Effects of Modified Qianjin Weijing Decoction on Lung Expression of SIGIRR Protein and Its m RNA in Rats with Chronic Obstructive Pulmonary Disease

作　　者：张海英[1] 杨爱东[1] 闫玲玲[1] 吴中华[1] 符胜光[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《辽宁中医药大学学报》2015年第2期19-22,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家中医药管理局重点学科项目(2009);上海中医药大学研究生创新能力培养专项项目(P2910302);上海中医药大学中医临床基础学科建设;名师研究室及基础医学院攀登计划项目(12ZLJ08;Z1301011310;C0010112);上海市教委预算内科研项目(2011JW89);上海市重点学科建设资助项目(S30301)

摘　　要：目的:探讨加味千金苇茎汤对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠肺组织单免疫球蛋白IL-1相关蛋白(single immunoglobulin IL-1 related protein,SIGIRR)及其m RNA表达的影响。方法:Wistar雄性大鼠随机分为正常组、模型组、地塞米松组和加味千金苇茎汤组(每组8只)。采用脂多糖气管滴注联合烟熏方法建立COPD大鼠模型,采用免疫组化ABC法和实时荧光定量聚合酶链反应检测SIGIRR蛋白及其m RNA表达,观察加味小青龙汤对上述指标以及肺组织病理形态学变化的影响。结果:与正常组比较,模型组肺组织SIGIRR蛋白表达明显降低(P<0.05);与模型组比较,地塞米松组和加味千金苇茎汤组肺组织SIGIRR蛋白及其m RNA的表达明显升高(P<0.01)。病理观察显示,模型组肺内细支气管上皮空泡变性坏死脱落,管壁结构破坏,大量炎细胞浸润,腔内炎性充满渗出物,地塞米松组和加味千金苇茎汤组仅见轻度肺间质炎症。结论:加味千金苇茎汤能够减轻慢性阻塞性肺疾病模型大鼠肺组织的损伤,对COPD模型肺组织有保护作用,其机制可能与其能够上调SIGIRR蛋白表达有关。Objective：To investigate the effects of Modified Qianjin Weijing Decoction（MQJWJD）on lung expression of SIGIRR（single immunoglobulin IL-1 related protein）and its m RNA in rats with chronic obstructive pulmonary disease（COPD）. Methods：Male Wistar rats were randomly divided into normal control group,model control group,MQJWJD group and dexamethasone group（n=8）. The COPD model of rats were bulit by the lipopolysaccharide tracheal instillation and smudging. The expression of SIGIRR protein and its m RNA were measured by immunohistochemical and real time PCR. While the histopathology of the lung tissue was observed by light microscope. Results：Compared with the normal group,the expression of SIGIRR protein was obviously decreased in the model group（P〈0.05）. Compared with the model group,the expression of SIGIRR protain and its m RNA were obviously increased in the dexamethasone group and AQJWJD group（P〈0.01）. Light microscope observation indicated that there were severe interstitial pneumonia,the destruction or disappearance of alveolar structure,the infiltration of the inflammatory cells,pulmonary interstitial fibrous tissue hyperplasia with great quantities in the model group while the pathological manifestations were much more ameliorated than those of the model group. The mild interstitial pneumonia was showed in the MQJWJD and the dexamethasone group. Conclusion：MQJWJD lessens the injury of lung tissue and has protective effects on COPD rats. The mechanism is possibly related to the up-regulatation of the expression of SIGIRR protein in COPD rats.

关 键 词：千金苇茎汤 慢性阻塞性肺疾病 SIGIRR 

分 类 号：R563[医药卫生—呼吸系统]