朝鲜白头翁皂苷类化学成分研究  

Studies on Saponins of Pulsatilla Eernua

作　　者：刘建宇[1,2] 马静[1,3] 宫益霞 周楠[1,3] 苏湘耘 樊文豪 许永男[1,2] 

机构地区：[1]教育部基于靶点的药物设计与研究重点实验室,辽宁沈阳110016 [2]沈阳药科大学制药工程学院,辽宁沈阳110016 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《辽宁中医药大学学报》2015年第2期45-48,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：教育部创新团队发展计划项目;辽宁省高校创新团队支持计划资助项目

摘　　要：目的:研究朝鲜白头翁Pulsatilla cernua(Thunb.)Bercht.et Opiz.根中皂苷类化学成分。方法:采用硅胶柱色谱、D101大孔吸附树脂、循环制备高效液相色谱等方法进行分离纯化,并通过化学方法和波谱手段鉴定化合物的结构。结果:从朝鲜白头翁干燥根70%乙醇提取物中分离得到10个化合物,分别鉴定为常春藤皂苷元3-O-α-L-吡喃阿拉伯糖苷(1),齐墩果酸3-O-α-L-吡喃阿拉伯糖基-28-O-β-D-吡喃葡萄糖酯苷(2),齐墩果酸3-O-α-L-吡喃鼠李糖基(1→2)-α-L-吡喃阿拉伯糖苷(3),常春藤皂苷元3-O-β-D-吡喃葡萄糖基(1→3)-α-L-吡喃鼠李糖基(1→2)-α-L-吡喃阿拉伯糖苷(4),齐墩果酸3-O-β-D-吡喃葡萄糖基(1→3)-α-L-吡喃鼠李糖基(1→2)-α-L-吡喃阿拉伯糖苷(5),齐墩果酸3-O-α-L-吡喃阿拉伯糖基-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡萄糖基(1→6)-β-D-吡喃葡萄糖酯苷(6),常春藤皂苷元3-O-α-L-吡喃鼠李糖基(1→2)-α-L-吡喃阿拉伯糖基-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡萄糖基(1→6)-β-D-吡喃葡萄糖酯苷(7),常春藤皂苷元3-O-β-D-吡喃葡萄糖基(1→4)-α-L-吡喃阿拉伯糖基-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡萄糖基(1→6)-β-D-吡喃葡萄糖酯苷(8),常春藤皂苷元3-O-α-L-吡喃鼠李糖基(1→2)-[β-D-吡喃葡萄糖基(1→4)]-α-L-吡喃阿拉伯糖基-28-O-β-D-吡喃葡萄糖基(1→6)-β-D-吡喃葡萄糖酯苷(9),常春藤皂苷元3-O-β-D-吡喃葡萄糖基(1→3)-α-L-吡喃鼠李糖基(1→2)-α-L-吡喃阿拉伯糖基-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡萄糖基(1→6)-β-D-吡喃葡萄糖酯苷(10)。结论:化合物2为首次从该属植物中分离得到,化合物9为首次从该植物中分离得到。Objective：To study the saponin constituents from the roots of Pulsatilla cernua. Methods：Compounds were isolated by silica gel chromatography,D101 macroporous adsorption resins and recycling preparative HPLC and their structures were elucidated on the basis of chemical and spectroscopic methods. Result：Ten chemical constituents were obtained and identified as hederagenin 3-O-α-Larabinopyranoside（1）,oleanolic acid 3-O-α-L-arabinopyranosyl-28-O-β-D-glucopyranosyl ester（2）,oleanolic acid 3-O-α-L-rhamnopyranosyl（1→2）-α-L-arabinopyranoside（3）,hederagenin 3-O-β-D-glucopyranosyl（1 → 3）-α-L-rhamnopyranosyl（1 → 2）-α-L-arabinopyranoside（4）,oleanolic acid 3-O-β-D-glucopyranosyl（1 → 3）-α-L-rhamnopyranosyl（1 → 2）-α-Larabinopyranoside（5）,oleanolic acid 3-O-α-L-arabinopyranosyl-28-O-α-L-rhamnopyranosyl（1→ 4）-β-D-glucopyranosyl（1 → 6）-β-D-glucopyranosyl ester（6）,hederagenin 3-O-α-Lrhamnopyranosyl（1 → 2）-α-L-arabinopyranosyl-28-O-α-L-rhamnopyranosyl（1 → 4）-β-Dglucopyranosyl（1→6）-β-D-glucopyranosyl ester（7）,hederagenin 3-O-β-D-glucopyranosyl（1→ 4）-α-L-arabinopyranosyl-28-O-α-L-rhamnopyranosyl（1 → 4）-β-D-glucopyranosyl（1→6）-β-D-glucopyranosyl ester（8）,hederagenin 3-O-α-L-rhamnopyranosyl（1→2）-[β-Dglucopyranosyl（1 → 4）]-α-L-arabinopyranosyl-28-O-β-D-glucopyranosyl（1 → 6）-β-Dglucopyranosyl ester（9）,hederagenin 3-O-β-D-glucopyranosyl（1→3）-α-L-rhamnopyranosy（1→ 2）-α-L-arabinopyranosyl-28-O-α-L-rhamnopyranosyl（1 → 4）-β-D-glucopyranosyl（1 →6）-β-D-glucopyranosyl ester（10）. Conclusion：Compound 2 was isolated from the plants in the genus Pulsatilla for the first time and 9 was isolated from this plant for the first time.

关 键 词：朝鲜白头翁 三萜皂苷 化学成分 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]