连翘解毒合剂质量标准研究  

Study on Quality Standard of Lianqiao Jiedu Mixture

作　　者：张娜[1] 赵海霞[2] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学附属医院,山东济南250011

出　　处：《辽宁中医药大学学报》2015年第2期49-51,共3页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的：建立医院制剂连翘解毒合剂的质量标准。方法：用薄层色谱法对连翘解毒合剂中的主要药味连翘、蒲公英、黄芩进行鉴别研究。采用高效液相色谱法（HPLC）测定连翘解毒合剂中主要有效成分绿原酸的含量。结果：TLC法可对连翘、蒲公英、黄芩进行鉴别,且斑点清晰,专属性较强,Rf值适中;HPLC测定结果显示,绿原酸含量在16.4-180.4μg范围内线性关系良好（r=0.9998,n=6）,平均回收率为98.45%（RSD=0.88%,n=6）。结论：本实验方法简便、准确、重现性好,可作为该制剂的质量控制方法,为连翘解毒合剂质量标准的提高提供科学依据。Objective：To establish the quality standard of Lianqiao Jiedu Mixture. Methods：Identifications of the main components of Lianqiao Jiedu Mixture including forsythia dandelion and Radix scutellariae were performed by TLC. The main effective components of chlorogenic acid were determined by HPLC. Results：Identifications of Fructus Forsythiae,dandelion,Radix Scutellariae of Lianqiao JieduMixture were established by TLC. And spots were clear with strong specificity and Rf value was moderate. The HPLC results showed that the linear range of chlorogenic acid was 16.4-180.4 μg（r=0.999 8,n=6）. The average recovery was 98.45% with RSD=0.88%（n=6）. Conclusion：These methods are simple,accurate and repeatable. They can be used as quality standard for Lianqiao Jiedu Mixture to provide scientific basis for the improvement of quality standards of Lianqiao Jiedu Mixture.

关 键 词：连翘解毒合剂 质量标准 薄层色谱法 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]