一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发  

Development of Molecular Markers Linked to a Novel QTL for TuMV-C4 Resistance in Brassica rapa L. ssp. pekinensis

作　　者：田希辉 于拴仓[2] 苏同兵 张凤兰[2] 余阳俊[2] 张德双[2] 赵岫云[2] 汪维红[2] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,北京100097

出　　处：《华北农学报》2014年第6期1-5,共5页Acta Agriculturae Boreali-Sinica

基　　金：科技部科技支撑项目(2012BAD50G01;2012BAD02B01);科技部"863"项目(2012AA020103;2012AA100103);国家大宗蔬菜产业技术体系(CARS-25-A-11)

摘　　要：为寻找与TuMV抗性基因紧密连锁的分子标记,选用高抗病毒病大白菜自交系91-112和高感病毒病自交系T12-19以及由二者为双亲构建的包含100个株系的DH群体为材料,通过SSR和InDel标记的遗传分析,在A09上定位了一个新的与大白菜苗期Tu MV-C4抗性相关的主效QTL位点BrTu A09。在此基础上,针对该QTL位点所在的标记区间,根据作图群体双亲的重测序结果,设计合成27对引物,其中11个In Del在双亲间具有多态性,且条带单一、扩增稳定;连锁分析发现,11个InDel标记均被定位在A09连锁群上BrTu A09的置信区间。利用BC1群体进行标记验证发现,这些标记对高抗单株选择的准确率均达到78%以上,可应用于分子标记辅助选择,为大白菜Tu MV抗病分子育种奠定了良好的基础。Our research constructed a molecular genetic map with a line 91-112（ highly resistant to Tu MVC4）,a highly susceptible line T12-19 and the DH population derived from microspore culture of F1（ 91-112 × T12-19）. A total of 45 markers were mapped to the A09 chromosome,and a new QTL-Br Tu A09 controlling Tu MV-C4 resistance was identified. On this basis,27 pairs of primers were designed by screening the In Del loci on A09 chromosome of Chinese cabbage based on genome re-sequencing. The results showed that distinct PCR amplification products were obtained from the In Del markers. Among these markers,11 In Del were polymorphic on the tested parent materials,and mapped to the A09 chromosome. By linkage analysis,these markers were tightly linked to the QTLBrtu A09. The studies showed that these markers can be applied in marker-assisted selection in Chinese cabbage hybrid breeding programs and improve the resistance to Tu MV-C4.

关 键 词：大白菜 QTL TUMV 分子标记 

分 类 号：Q78[生物学—分子生物学] S634.3[农业科学—蔬菜学]