水稻中脉缺陷突变体dl-6的遗传分析与精细定位  

Genetic Analysis and Gene Fine Mapping of a Midrib-deficient Mutant dl-6 in Rice

作　　者：王军[1] 王婧[2] 杨杰[1] 赵祥强[2] 朱金燕[1] 范方军[1] 李文奇[1] 王芳权[1] 仲维功[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,国家水稻改良中心南京分中心,江苏南京210014 [2]南通大学生命科学院,江苏南通226007

出　　处：《华北农学报》2014年第6期6-10,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划重大项目(2011BAD16B03);江苏省研究生创新工程项目(CXZZ12_0906);江苏省农业自主创新资金项目(CX(12)1003);江苏省自然科学基金项目(BK20130725);江苏省科技支撑计划项目(BE2012309)

摘　　要：为了克隆DL-6基因,以dl-6与9311配制正反杂交组合进行性状分析发现,dl-6突变性状受1对隐性核基因控制,利用SSR分子标记将控制dl-6性状的基因DL-6初步定位在水稻第3染色体的短臂上,进一步利用新发展的In Del标记将DL-6基因精细定位在I3-5和I3-8之间85 kb的物理距离内。对该区段内存在的开放阅读框(ORF)进行分析,发现其中ORF9编码的YABBY基因是一个与叶脉发育相关的基因,对突变体dl-6和野生型中YABBY基因进行测序,将测序结果与数据中日本晴序列进行比对发现,突变体dl-6中YABBY基因的第1个外显子存在1个单碱基突变,该突变导致野生型中编码的半胱氨酸突变为突变体中的精氨酸;同时突变体dl-6在YABBY基因的3'端还存在8个碱基的缺失。这2个突变位点哪个是导致dl-6突变的功能区目前还不确定,有待进一步研究。To clone the DL-6 locus,positive and negative cross combinations were developed between dl-6 and9311,and genetic analysis found that drooping leaf was controlled by one recessive nuclear gene DL-6. DL-6 was primarily mapped on the short arm of chromosome 3 with SSR markers. With newly developed markers DL-6 was finally fine mapped in 85 kb region between markers I3-5 and I3-8. Open reading frame（ ORF） analysis revealed that ORF9 might be a drooping leaf related gene. ORF9 both in mutant dl-6 and in wild type were sequenced and 1bp mutation was found in first exon in dl-6 when blasted with Nipponbare. Cysteine mutated into arginine in dl-6.Meanwhile,8 bp deletion was also found at 3＇ end of YABBY. These two mutations which one is the functional mutation of DL-6 is still uncertain and need deeply research.

关 键 词：水稻 中脉缺陷 基因定位 dl-6 

分 类 号：Q78[生物学—分子生物学] S511.03[农业科学—作物学]