桃PG基因启动子克隆及序列分析  

Cloning and Sequence Analysis of a Novel Promoter of Polygalacturonase Gene in Pruns persica( L. )

作　　者：陈迪新[1] 李艳梅[1] 郭国宁[1] 郗慧[1] 杨瑞娟 杨英军[1] 

机构地区：[1]河南科技大学林学院,河南洛阳471003

出　　处：《华北农学报》2014年第6期52-57,共6页Acta Agriculturae Boreali-Sinica

基　　金：河南省教育厅重点项目(13A210873);洛阳市科技发展计划项目(1402011A)

摘　　要：为获得桃多聚半乳糖醛酸酶（PG）基因启动子并分析调控元件,以桃品种大久保基因组DNA为模板,采用染色体步移技术克隆了PG基因的部分序列及其5＇侧翼区,并分析其调控元件组成。结果表明,克隆产物长986 bp,3＇侧翼序列与Gen Bank中的PG基因序列的5＇端部分序列同源性为96%,登录号为FJ940722。在789-839 bp位置存在基础启动子序列,转录起始位点位于828 bp的碱基C,其可能性为0.98。除含有CAAT-Box、TATA-Box等基本启动子元件外,还存在众多参与光响应元件、干旱诱导MYB结合位点、水杨酸响应元件等应答激素和胁迫信号元件,表明PG基因的转录和表达可能受到激素、干旱、光照等外界环境的胁迫以及衰老的调控。The objective of the study was to obtain the 5＇ flanking sequence of polygalacturonase gene from the genomic DNA of Pruns persica（ L.） Batsch. Chromosome walking techniques and the software of PROMOTER PREDICTION and PLANT CARE online were used. The results showed that a novel promoter of PG gene was obtained,the length of it was 986 bp,alignment indicated that the sequence had 96% homology with that of the polygalacturonase gene. The core promoter regions and some upstream regulatory elements in this fragment were analyzed. Transcriptional start site（ TSS） was C. The sequence of PG gene promoter contained several specific acting elements,They were TATA-box,CAAT-box,light-responsive elements,drought-induced MYB binding sites,salicylic acid response elements,and other cis-elements. The accession number was FJ940722. The studying suggested that the expression of the novel promoter of PG gene could be regulated by hormone,drought,light and other factors.

关 键 词：桃 多聚半乳糖醛酸酶 启动子 序列分析 

分 类 号：S667.9[农业科学—果树学] Q78[农业科学—园艺学]