AY9944-A-7对于IBMX抑制的绵羊卵母细胞体外成熟的诱导作用  

Roles of AY 9944-A-7 in Inducing in Vitro Maturation of Sheep Oocytes Inhibited by IBMX

作　　者：郝瑞荣[1] 吕丽华 高俊杰[2] 张春香[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]山西农业大学园艺学院,山西太谷030801

出　　处：《华北农学报》2014年第6期73-77,共5页Acta Agriculturae Boreali-Sinica

基　　金：转基因生物新品种培育重大专项(2011ZX08008-003);山西农业大学引进人才科研启动经费项目(2013YJ15)

摘　　要：为了探究AY 9944-A-7在3-异丁基-1-甲基黄嘌呤（3-isobutyl-1-methylxanthine,IBMX）阻滞的绵羊卵母细胞减数分裂恢复过程中的作用,将绵羊卵丘-卵母细胞复合体（COCs）、分离的卵丘-卵母细胞（split COCs）和脱卵丘后制成的裸卵（DOs）培养于含250μmol/L IBMX的体外成熟液中,分别添加10,20,30,40μmol/L的AY 9944-A-7以诱导卵母细胞恢复减数分裂。结果表明,10-40μmol/L的AY 9944-A-7极显著地提高了绵羊COCs的生发泡破裂（GVBD）率和第一极体（PBI）排出率（P〈0.01）;添加AY 9944-A-7对IBMX阻滞的绵羊DOs生发泡破裂和PBI排出没有显著的刺激作用（P〉0.05）;40μmol/L AY 9944-A-7使绵羊COCs、split COCs、DOs的退化率极显著提高（P〈0.01）。说明AY 9944-A-7能够诱导IBMX抑制的绵羊COCs恢复减数分裂成熟,但是过量的AY 9944-A-7对绵羊卵母细胞有毒性,AY 9944-A-7诱导绵羊COCs克服IBMX抑制恢复减数分裂成熟的适宜剂量为20μmol/L。To evaluate the effects of AY 9944-A-7 on 3-isobutyl-1-methylxanthine（ IBMX）-arrested meiotic maturation in ovine oocytes,sheep cumulus oocyte complexes（ COCs）,split COCs and denuded oocytes（ DOs）were cultured in medium supplemented with 250 μmol / L IBMX. Different concentrations of AY 9944-A-7（ 0,10,20,30,40 μmol / L） were added into maturation medium respectively to induce meiotic resumption of oocytes. The results showed that,AY 9944-A-7 supplementation in the range from 10 to 40 μmol / L increased the percentage of GVBD and the rate of PBI extrusion significantly in ovine COCs（ P〈0. 01）. While AY 9944-A-7 showed no significantly stimulatory effect on the percentage of GVBD and the rate of PBI extrusion of ovine DOs arrested by IBMX（ P〈0. 05）. And the highest dose tested（ 40 μmol/L） resulted in significant degenerations in ovine COCs,split COCs and DOs（ P〈0. 01）. The results indicated that AY 9944-A-7 induced meiotic resumption inhibited by IBMX in sheep COCs. But AY 9944-A-7 over dose showed toxic effects on ovine oocytes. The optimal dosage for AY 9944-A-7 to overcome the meiotic inhibition by IBMX and resume meiosis was 20 μmol / L.

关 键 词：绵羊 卵母细胞 AY9944-A-7 3-异丁基-1-甲基黄嘌呤(IBMX) 

分 类 号：S813.1[农业科学—畜牧学]