红芝漆酶高产菌株的诱变选育  

Mutational Screening of Ganoderma lucidum Strain with High Yield of Laccase

作　　者：易小畅[1,2] 蒋智[1,2] 李娜[1,2] 陆兵[1,2] 谌斌[1,2] 

机构地区：[1]广西环境工程与保护评价重点实验室,广西桂林541004 [2]广西师范大学环境与资源学院,广西桂林541004

出　　处：《食品研究与开发》2014年第24期139-142,共4页Food Research and Development

基　　金：广西自然科学基金面上项目(2011GXNSFA018080);广西研究生教育创新计划项目(YCSZ2014091);广西环境工程与保护评价重点实验室平台建设项目(二期)

摘　　要：以药用真菌红芝(Ganoderma lucidum)为出发菌,通过紫外线(Ultraviolet ray,UV)、亚硝基胍(Nitrosoguanidine,NTG)及紫外线(UV)-亚硝基胍(NTG)逐级诱变处理其菌丝体片段,采用平板变色法初筛、愈创木酚法测定发酵液漆酶酶活力复筛,获得1株漆酶高产诱变菌株LYL263。用复筛液体培养基震荡培养时,其平均酶活力比出发菌株提高187%,达到145 500 U/L,且产酶稳定。The mycelium fragments of Ganoderma lucidum were exposed successively under ultraviolet ray, nitrosoguanidine, ultraviolet ray-nitrosoguanidine and the mutants were screened in guaiacol-containing SPDA plates, and then the laccase activities of the mutated strains were determined by guaiacol. A mutant LYL263 with high yield of laccase was obtained. The average activity of laccase from LYL263 had increased up to 145 500 U/L, and its ability of producing laccase was stable on culture condition of liquid medium with glucose and yeast extract.

关 键 词：红芝 组成型漆酶 紫外诱变 亚硝基胍诱变 

分 类 号：Q933[生物学—微生物学]