绿贻贝贝壳的微结构特征及光谱分析  

Microstructural Characteristics and FTIR Analysis of the Shell from Green Mussel(Perna canaliculus)

作　　者：鲍林飞 高鹏[1] 赵鲁苹 徐焕志[1] 陈东[2] 廖智[1] 

机构地区：[1]浙江海洋学院海洋科学与技术学院,海洋生物资源及分子工程实验室,浙江舟山316022 [2]浙江海洋学院石油与能源工程学院,浙江舟山316022

出　　处：《浙江海洋学院学报（自然科学版）》2014年第4期347-353,共7页Journal of Zhejiang Ocean University(Natural Science Edition)

基　　金：浙江省自然科学基金项目(LY14C100001)

摘　　要：贻贝贝壳是一种由碳酸钙晶体和少量有机质形成的高度有序的天然纳米复合材料,历来是生物材料、组织工程以及仿生学研究的重要对象。利用扫描电子显微镜、傅里叶红外光谱分析以及X射线衍射分析等,对绿贻贝贝壳的微观结构、碳酸钙晶体构型以及有机质结构特征进行分析。绿贻贝贝壳主要包含珍珠质(Nacre)层和肌棱柱层;两者的碳酸钙晶体构型均采取了文石构型;在后闭壳肌-贝壳连接部位,肌肉与贝壳碳酸钙晶体之间由一层有机质膜连接且该膜质地明显区别于珍珠质层表面的膜。绿贻贝各层结构中的蛋白质均以β-折叠为优势构象,但是肌棱柱层中蛋白质的β-折叠含量较珍珠质层高。For understanding the microstructural characteristics of mussel shell correlating to muscle-shell attachment, the microstrcuture, polymorph, and organic matrix of adductor muscle attached scar （AMS） and non-muscle attached zone in the adult Perna canaliculus shell were investigated and compared. The adductor muscle scar has a hierarchically complex multilayer composed of organic matrix and two distinct mineral lay-ers, including nacre and myostracum layer. Fourier Transform Infrared Spectroscopy （FTIR） and X-Ray Diffrac-tion （XRD） results showed that the inorganic compounds of both nacre and myostracum layer are composed of aragonite. The adductor muscle attaches to the myostracum layer through an organic film and forms a muscle-film-shell junction. The film adheres firmly to the myostracum layer surface and shows a very smooth texture, which is deferent with the film on nacre surface. The amide region of FTIR spectra from decalcified shell sam-ples was analyzed and we showed that the shell proteins in vivo are in the higher structural ordered state, andβ-parallel structure was predominant in both shell layer.

关 键 词：绿贻贝 贝壳 扫描电子显微镜 傅里叶红外光谱 X射线衍射 

分 类 号：TB332[一般工业技术—材料科学与工程] O657.33[理学—分析化学]