新生期大鼠重复吸入七氟烷对其成年期Aβ、Tau蛋白的影响  

Effects of repeatedly-inhaled sevoflurane in the neonatal period on Tau and amyloid beta protein expressions in adulthood

作　　者：马欢欢[1] 张超[1] 郑淑文[1] 朱昭琼[1] 刘进[2] 

机构地区：[1]遵义医学院麻醉学系,贵州遵义563099 [2]四川大学华西医院麻醉科,四川成都610041

出　　处：《遵义医学院学报》2015年第1期60-63,共4页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:81360180);山东鲁南制药集团资助项目(NO:遵鲁药[2010]001)

摘　　要：目的研究新生期大鼠间断、重复吸人七氟烷，对其成年期行为学及β淀粉样蛋白（Aβ）、异常磷酸化Tau蛋白（PHF—Tau）在海马CA1区的表达。方法7日龄SD大鼠24只，随机分为3组（n=8），1．5％七氟烷组（S1组），2．6％七氟烷组（S2组），对照组（C组）。分别于出生7、14、21d（P7、P14、P21）吸入1．5％、2．6％七氟烷及运载气体1h，于P91～P95行定位航行和空间探索实验。水迷宫实验结束后，取海马组织，用免疫组织化学的方法测定CA1区Aβ、PHF—Tau蛋白的表达。结果①与C组相比，P94，S1组、S2组逃避潜伏期延长（P〈0．01或P〈0．05），P95，S1组潜伏期延长（P〈0．05）；②与P91相比，C组、S2组P94和P95逃避潜伏期缩短（P〈0．05）；S1组，P93逃避潜伏期缩短（P〈0．05）；③与C组比较，s1组原平台象限滞留时间百分比减少（P〈0．01）；④与C组比较，S1组PHF—Tau蛋白MOD增加（P〈0．05），S1组、S2组Ap蛋白MOD增加（P〈0．05或P〈0．01）。结论新生期大鼠间断、重复吸入1．5％、2．6％的七氟烷可使其成年期学习记忆能力下降，其中吸入1．5％七氟烷较2．6％七氟烷影响更持久。其机制可能与成年期海马CA1区AB、PHF-Tau蛋白水平的改变有关。Objective To study the effects of interrupted and repeated inhalation of sevoflurane in neonatal rats on praxiology of adulthood and the expressions of amyloid beta - protein （Aβ） and abnormal phosphorylated Tau （ PHF - Tau） protein in the CA1 area of hippocampus. Methods Twenty - four Seven - day old neonatal SD rats were divided into 3 groups randomly （n = 8 in each group）, 1.5% sevoflurane （group S1 ）, 2.6% sevoflurane （group S2 ） and the control group （ group C）. Rats in each group were inhalated 1.5%, 2.6% sevoflurane and carrier gas for 1 h in the 7th（ P7 ） , 14th（P14）, 21th day （P21）, respectively. Rats were tested via a water maze experiment in P91 -P95 and the escape latency and retention time in the original platform quadrant and the per- centage of entering times in spatial probe test were recorded. The hippocampus afer morris water maze test was collected to determine the expressions of Aβ and PHF - Tau protein with immunohistochemical method. Results 1 ）Compared with group C, escape latencies in groupS1 and group S2 were prlonged （P 〈 0.01 or P 〈 0.05 ） in the 94th day and in group S1 in the 95th day （P 〈0.05） ;2）The escape latency was decreased in P94 and P95 of group C and group S2 and P93 of group S1 compared with that in P91 （P 〈 0.05 ） ;3 ）Compared with group C, the percentage of retention time in the original platform quadrant was significantly reduced in group S1 （P 〈 0.01 ） ;4） Compared with group C, the mean opticaldensity （MOD） of PHF - Tau protein in group S1 （P 〈0.05 ） and Aβ in group S1 and S2 （P 〈 0. 01 or P 〈 0.05 ） was increased. Conclusion The interrupted and repeated inhalation of 1.5% and 2.6% sevoflurane on neonatal rats could impair learning and memory in adulthood, in which the effects of inhalation of 1.5% sevoflurane lasted longer than those of 2.6% sevoflurane. The mecha- nisms might be related to the deposition of Aβ protein and changes of the PHF- Tau protein expression.

关 键 词：七氟烷 学习记忆 AΒ蛋白 磷酸化TAU蛋白 

分 类 号：R614.2[医药卫生—麻醉学]