基质辅助激光解吸电离飞行时间质谱技术在临床微生物鉴定中的应用  被引量:11

Matrix assisted laser desorption ionization time of flight mass spectrometry for microbial identification in clinical microbiology

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作  者:Marc van Nuenen Kirk Doing 顾兵 Alex van Belkum 

机构地区:[1]Mass Spectrometry, Institut Merieux [2]Clinical Affairs Durham, Institut Merieux [3]南京医科大学第一附属医院检验学部 [4]R&D Microbiology, Institut Merieux

出  处:《中华检验医学杂志》2015年第1期64-66,共3页Chinese Journal of Laboratory Medicine

基  金:国家自然科学基金(81000754,81471994)

摘  要:近年来,基质辅助激光解吸电离飞行时间质谱技术(MALDI—TOF—MS)已从物理实验室走进临床微生物实验室,用于微生物的鉴定,正逐步取代由Pasteur和Koch建立的基于细菌表型的传统鉴定方法。许多欧洲微生物实验室目前正在使用MALDI-TOF-MS,大大提高了工作效率。鉴于美国食品药品管理局已经批准其用于体外诊断,MALDI-TOF-MS将很快在美国普及应用。这也将进一步推动了质谱技术在微生物药敏和流行学分型中的发展和应用。本文综述了MALDI—TOF—MS在临床微生物实验室的应用现状,并通过多中心临床试验结果,比较不同MALDI—TOF-MS仪器获得的诊断数据,重点阐述其在临床微生物领域的相关研究和应用。Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF-MS) recently moved from physics laboratories into diagnostic clinical microbiology. In this capacity it is currently replacing phenotypie bacterial identification tools that were designed and developed by Pasteur and Koch. Many European microbiology laboratories now use MALDI-TOF-MS with a high degree of efficiency. In the USA this will soon become common as well since the Food and Drug Administration (FDA) has now cleared the first of the commercially available systems for in vitro diagnostic use. This will also further spark the development of mass spectrometry applications in the field of antimierobial susceptibility testing and epidemiological typing. This review summarizes the current state of affairs with respect to MALDI-TOF-MS in clinical microbiology. Emphasis will be on clinical relevance and studies in that field and the diagnostic data obtained through comparative analyses of different MALDI-TOF-MS instrumentation and through muhi-center validation studies will be reviewed.

关 键 词:光谱法 质量 基质辅助激光解吸电离 微生物学技术 

分 类 号:R446.5[医药卫生—诊断学]

 

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