聚乙二醇-金纳米棒介导的近红外光热抑菌作用  

Near-IR Photothermal Antibacterial Effects of Polyethylene Glycol(PEG) Modified Gold Nanorods

作　　者：冯晓燕[1,2,3,4] 陈莹[1,2,3,4] 刘玉鹏[1,2,3,4] 王春鹏[1,2,3,4] 储富祥[1,2,3,4] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,南京210042

出　　处：《无机化学学报》2015年第2期215-221,共7页Chinese Journal of Inorganic Chemistry

基　　金：中央级公益性科研院所基本科研业务费专项资金(No.CAFINT2010K04);国家自然科学基金(No.31100427);江苏省自然科学基金(No.BK20131071)资助项目

摘　　要：种子生长法合成纵向表面等离子体共振吸收峰为785 nm的金纳米棒,并对其表面进行聚乙二醇(PEG)修饰,研究了表面修饰PEG的金纳米棒(polyethylene glycol modified gold nanorods,PEG-GNR)的光热转化效应,并测试了其细胞毒性。以革兰氏阳性菌金黄色葡萄球菌、蜡状芽孢杆菌,革兰氏阴性菌大肠埃希氏菌及铜绿假单胞菌为细菌模型,详细研究了PEG-GNR在808nm波长近红外激光照射下金纳米棒浓度和照射功率对抑菌效果的影响。结果表明,PEG-GNR对革兰氏阳性菌和革兰氏阴性菌在近红外照射下均有较好的抑菌效果,并且抑菌效果与金纳米棒的浓度和照射功率有着密切的关系;结合荧光显微镜和透射电子显微镜对细菌坏死状况的观察,初步证实细菌对PEG-GNR有效吸收是近红外光热杀菌的关键因素。Gold nanorods （GNR） with longitudinal surface plasma resonance （LSPR） absorption at 785 nm were synthesized by seed-mediated growth method and their surface was modified with polyethylene glycol （PEG） macromolecular （PEG-GNR）. The photothermal conversion effect and cytotoxicity of PEG-GNR were investigated. Different bacteria, including gram-positive bacterium Staphylococcus aureus and Bacillus cereus, gram-negative bacterium Escherichia coli and Pseudomonas aeruginosa were used to analyze the influences of concentration of PEG-GNR and laser output power on the inhibition effects. The results show that the PEG-GNR has good antibacterial properties for both Gram positive and negative bacterium under the radiation of near-IR laser. The concentration of PEG-GNR and laser output power determined antibacterial effects of the PEG-GNR. The preliminary investigation on the antibacterial mechanism was explored by studying of bacteria apoptosis status with fluorescence microscope and transmission electronic microscope, suggesting that the effective absorption of the PEG-GNR by the cells is one of the key factors in the process of photothermal antibiosis.

关 键 词：金纳米棒 PEG修饰 光热抑菌 

分 类 号：TQ131.2[化学工程—无机化工]