头针配合康复训练对脑缺血再灌注损伤沙鼠模型神经可塑性相关蛋白MAP-2的调节作用  

The effect of scalp acupuncture combined with rehabilitation training on the regulation of neuro-plasticity-associated protein MAP-2 in gerbils modelling cerebral ischemia and reperfusion

作　　者：郭效德[1] 周莹[2] 王睿[3] 周翔[3] 郭银丰[3] 唐君[3] 高山[3] 

机构地区：[1]山东泰山医学院附属莱芜医院针灸推拿科,莱芜271199 [2]山东泰山医学院附属莱芜医院康复理疗科,莱芜271199 [3]杭州市中医院推拿科

出　　处：《中华物理医学与康复杂志》2015年第1期7-10,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的 观测头针配合康复训练对脑缺血再灌注损伤沙鼠模型神经可塑性相关蛋白MAP-2调节作用.方法 使用SPSS 19.0版统计软件生成的随机数字表将36只成功造模的脑缺血再灌注损伤模型雄性沙鼠随机分为模型组、康复训练组和头针配合康复训练组,每组12只.模型组不作任何干预措施,康复训练组采用康复训练治疗,头针配合康复训练组给予头部穴位电针刺激配合康复训练治疗;1日1次,共治疗14 d.分别于术后24 h、第7天和第14天,对各组沙鼠采用Bederson评分评定其神经功能,14 d后行缺血灶MAP-2表达检测,并进行统计学分析比较.结果 术后第7天,头针配合康复训练组的Bederson评分为（1.81±0.52）分,与模型组[（2.13±0.49）分]和康复训练组[（2.00±0.31）分]比较,组间差异均无统计学意义（P＞0.05）;但第14天头针配合康复训练组的Bederson评分为（0.47±0.31）分,与模型组[（1.46±0.72）分]和康复训练组[（1.04±0.63）分]比较,头针配合康复训练组明显优于其余两组（P＜0.05）,且康复训练组亦优于模型组（P＜0.05）.MAP-2蛋白表达水平定量分析,头针配合康复训练组为（0.91±0.18）,与模型组的（0.43±0.21）及康复训练组的（0.67±0.24）比较,组间差异均有统计学意义（P＜0.05）,康复训练组较模型组也有一定优势（P＜0.05）.结论 头针配合康复训练能更好地促进神经运动功能的恢复,并增加脑缺血再灌注损伤沙鼠缺血灶神经可塑性相关蛋白MAP-2的表达.Objective To observe the effect of scalp acupuncture and rehabilitation training on the regulation of neuro-plasticity-associated protein MAP-2 in gerbils modelling cerebral ischemia and reperfusion.Methods Cerebral ischemia and reperfusion were modelled into thirty-six male gerbils.They were then randomly divided into a model group （A）,a scalp acupuncture and rehabilitation training group （B） and a rehabilitation training group （C）,each of 12 cases.The scalp acupuncture and rehabilitation training were administered daily to the gerbils in group B beginning 24 h after modeling,for 14 d.Any changes in Bederson scores were observed after one,7 and 14 days.On the 14th day the expression of MAP-2 around the infarct focus was detected using western blotting in all 3 groups.Results On the 7th day the average Bederson score in group B （1.81 ± 0.52） was not significantly different from that in group A （2.13 ± 0.49） or group C （2.00 ± 0.31） （P 〉 0.05）.At the end of the treatment,however,there were significant differences between the groups,with group B （0.47 ± 0.31） scoring significantly better than groups C （1.04 ± 0.63） and A （1.46 ± 0.72） （P 〈 0.05）.Group C was also significantly better than group A （P 〈0.05）.The expression of MAP-2 as measured as integrated optical intensity （IOD） in group B （0.91 ±0.18） was significantly higher than that in group A （0.43 ± 0.21） and group C （0.67 ± 0.24） （P 〈 0.05） ; so was group C compared to group A （P 〈0.05）.Conclusion The scalp acupuncture combined with rehabilitation training can improve the recovery of motor function and enhance the expression of neuro-plasticity-associated protein MAP-2 in gerbils with after cerebral ischemia and reperfusion.

关 键 词：脑缺血再灌注损伤 头针 MAP-2 沙鼠 针灸 Microtubule-associated protein 2 

分 类 号：R245[医药卫生—针灸推拿学]