308 nm准分子激光对白癜风豚鼠模型Treg及Th17细胞的影响  

Effect of 308 nm laser irradiation on Treg cells and Th17 cells in guinea pigs modelling vitiligo

作　　者：孟丽亚[1] 王逸飞[2] 魏国[1] 张春红[1] 刘瑛[1] 史永俭[1] 张春敏[1] 

机构地区：[1]山东大学第二医院皮肤科,济南250033 [2]山东大学医学院

出　　处：《中华物理医学与康复杂志》2015年第1期11-15,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：山东大学第二医院院青年基金（Y2013010076）,山东省科技发展计划（2014GSF118147）

摘　　要：目的 观察308 nm准分子激光治疗白癜风豚鼠模型的疗效及对Treg、Th17细胞的影响.方法 采用5％对苯二酚（氢醌）涂抹豚鼠局部皮肤制备实验性白癜风豚鼠模型,待制模成功后将其分为实验组及模型组,同时选取正常豚鼠纳入正常对照组.采用308 nm准分子激光对实验组白癜风豚鼠皮损处进行照射,模型组及正常对照组豚鼠饲养期间均未给予其他特殊干预.于激光照射8周后观察各组豚鼠白斑恢复情况并进行疗效评定,应用实时荧光定量PCR技术检测各组豚鼠Treg、Th17细胞特异性相关因子Foxp3、IL-17 mRNA表达;应用免疫组化法检测各组豚鼠皮损组织中Foxp3、IL-17表达.结果 本研究中实验组白癜风豚鼠模型治疗有效率为100％.RT-PCR结果显示,实验组及模型组Foxp3 mRNA表达[分别为（0.33 ±0.03）和（0.02±0.07]均较正常对照组明显增高（均P＜0.05）,并且实验组Foxp3 mRNA表达亦显著强于模型组（P＜0.05）;实验组及模型组IL-17 mRNA表达[分别为（0.21 ±0.05）和（0.94 ±0.06]均较正常对照组明显增高（均P＜0.05）,并且模型组IL-17 mRNA表达亦显著强于实验组（P＜0.05）.免疫组化检查结果显示,实验组皮损组织表皮可见散在分布IL-17和Foxp3蛋白表达,模型组皮损组织表皮则出现大量IL-17蛋白表达.结论 308 nm准分子激光可通过调节Treg细胞和Th17细胞间免疫平衡,从而对白癜风豚鼠发挥治疗作用.Objective To assess the efficacy of 308 nm excimer laser irradiation for the treatment of guinea pig model of vitiligo and its immunoregulatory effect on Treg cell and Th17 cell.Methods Vitiligo was induced in guinea pigs by hydroquinone bleaching,and they were randomly divided into an experimental group and a control group.There was also a normal control group.The experimental group was treated with a 308 nm excimer laser at the nidus of vitiligo; the two control groups were given no intervention.After 8 weeks of treatment,leukasmus of the different groups were compared.The mRNA expression of Foxp3 and IL-17 were measured by real-time fluorescence quantitative （RTFQ-PCR） and the expression of Foxp3 and IL-17 in the lesions were measured by immunohistochemistry staining.Results The effectiveness rate was 100% in the experiment group.The results of RTFQ-PCR showed that the mRNA expression of Foxp3 in the experimental group and the vitiligo control group was significantly higher than the healthy control group （all P 〈 0.05）,being 0.33 ± 0.03 and 0.02 ±0.07 respectively,as was the mRNA expression of IL-17,being 0.21 ± 0.05 and 0.94 ± 0.06 accordingly; and the mRNA expression of IL-17 in the experimental group was significantly lower than the vitiligo control group（P 〈 0.05）.The immunohistochemical staining showed that Foxp3 and IL-17 protein was scattered in the lesions of the experimental group,while they were crowded in those of the vitiligo control group.Conclusion Laser irradiation might contribute to a therapeutic effect on vitiligo through modulating the balance of Treg and Th17 cells,at least in guinea pigs.

关 键 词：308 nm准分子激光 白癜风 TREG细胞 TH17细胞 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]