液态氟碳纳米脂质微粒与全氟丙烷纳米脂质微泡基波及对比脉冲序列成像模式下体外显影特性比较  

Acoustic enhancement with fundamental imaging and CPS imaging： comparison between nano-scale PFOB lipidic particles and C3F8 lipidic microbubbles, a vitro experimental study

作　　者：刘莹莹 谢明星[2] 徐金锋 王新房[2] 吕清[2] 张丽[2] 覃小娟[2] 项飞翔[2] 张艳容[2] 丁楠[3] 杨畅[3] 项光亚[3] 

机构地区：[1]暨南大学第二临床医学院,深圳市人民医院超声科,深圳518020 [2]华中科技大学同济医学院附属协和医院超声影像诊断科 湖北省分子影像重点实验室 [3]华中科技大学同济医学院药学院

出　　处：《中华超声影像学杂志》2015年第1期74-79,共6页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金面上项目（83071280）;深圳市科技创新委员会重大项目（JCYJ20130402092657773）

摘　　要：目的比较自制液态氟碳（PFOB）纳米脂质微粒造影剂与全氟丙烷（C3F8）纳米脂质微泡造影剂在基波及对比脉冲序列成像（CPS）模式下的体外显影特性。方法分别制备生物素化（以生物素标记外膜）及空白（外膜未标记生物素）的PFOB脂质微粒造影剂，生物素化及空白的C3F8脂质微泡造影剂。比较生物素化及空白PFOB脂质微粒、生物素化及空白C3F8脂质微泡在加入亲和素前、后粒径变化情况。以高频探头分别在基波及CPS两种成像模式下观察各组造影剂加入亲和素前、后的显影效果，并运用METLAB软件分析比较。结果①空白PFOB脂质微粒及C3F8脂质微泡加入亲和素前、后粒径均无明显变化，生物素化PFOB脂质微粒及C3F8脂质微泡加入亲和素后粒径均较加入前明显增大（P〈0．05）。②基波成像模式下，加人亲和素前，空白及生物素化PFOB脂质微粒则均未见明显显影，而空白及生物素化C3F8脂质微泡均有较好的显影效果；加入亲和素后，生物素化PFOB脂质微粒显影明显增强（P〈0．05），而空白PFOB脂质微粒及两组C3F8脂质微泡显影情况无明显改变。③CPS成像模式下，生物素化PFOB脂质微粒造影剂加入亲和素前、后均未见明显信号显示，两者间信号强度无明显差异；而生物素化C3F8脂质微泡造影剂加入亲和素前、后均可见良好的显影效果，两者的信号强度亦无明显差异。结论比较C3F8脂质微泡，PFOB纳米脂质微粒粒径可控性更好，且具有更高的靶向／非靶向信噪比，更符合靶向超声造影剂的要求。Objective To prepare two kinds of nano scale ultrasound contrast agents,perfluorooctyl bromide（PFOB） lipidic particles and perfluoropropane （C3F8） lipidic microbubbles, and compare their characteristics of acoustic enhancement with fundamental imaging and contrast pulse sequence （CPS） imaging in vi＇tro. Methods Physico-chemical properties of PFOB lipidic particles and C3F8 lipidic microbubbles were measured, including morphous, size distribution, Zeta potential, concentration and stability. PFOB lipidic particles with and without biotin, C3F8 lipidic microbubbles with and without biotin were prepared, respectively. Size distribution of four contrast agents were measured, before and after adding avidin. Then, four kinds of ultrasound contrast agents were imaged with both fundamental and CPS imaging before and after avidin was added. Signal intensities were compared using METLAB software. Results （i） Addition of avidin had no effect on particle diameters of control lipidic particles/microbubbles. However,the particle diameters of biotinylated lipidic particles and microbubbles were both enlarged with the addition of

关 键 词：超声检查 微气泡 脂质体 液态氟烷 

分 类 号：R981.1[医药卫生—药品]