HP1188-IgY对感染幽门螺杆菌BALB／c小鼠的治疗作用  

Treatment of Helicobacter pylori-infected gastritis in BALB/c mice by HP1188-IgY

作　　者：韩飞[1] 杨致邦[2] 李建英[1] 周政[1] 彭方毅[3,4] 姜海蓉[3] 杜红心[1] 

机构地区：[1]重庆三峡中心医院检验科,重庆404100 [2]重庆医科大学基础医学院病原生物学教研室,重庆400016 [3]重庆理工大学药学与生物工程学院制药工程专业,重庆400054 [4]重庆市江津区中心医院科教部,重庆402260

出　　处：《中国病理生理杂志》2015年第1期148-153,共6页Chinese Journal of Pathophysiology

基　　金：重庆市万州区科委重点科技计划(No.201203006)

摘　　要：目的:评价抗幽门螺杆菌(Helicobacter pylori,H.pylori)HP1188蛋黄抗体(HP1188-immunoglobulin yolk,HP1188-Ig Y)对BALB/c小鼠胃内H.pylori感染的治疗效果。方法:建立H.pylori感染BALB/c小鼠的动物模型,将建模成功的80只小鼠随机分为8组,每组10只。第1组(抗生素治疗组)、第2组(1 mg HP1188-Ig Y)、第3组(1 mg HP1188-Ig Y+30%硫糖铝)、第4组(5 mg HP1188-Ig Y)、第5组(5 mg HP1188-Ig Y+30%硫糖铝)、第6组(PBS对照组)和第7组(30%硫糖铝对照组)分别连续治疗10 d,每天1次;第8组间隔48 h皮下注射2.5 mg HP1188-Ig Y,共2次;另10只正常小鼠为第9组(健康对照组)。处理完成2周后取各组小鼠胃组织作H.pylori培养、快速尿素酶试验、PCR检测H.pylori特异性vac A和cag A及病理组织学检查,观察H.pylori清除情况并进行评分。结果:在小鼠体内,灌胃(1 mg HP1188-Ig Y+30%硫糖铝)即可有效治疗H.pylori感染引起的胃黏膜损害,其治疗效果与抗生素相似。结论:成功构建了H.pylori感染的BALB/c小鼠模型,选择30%硫糖铝为抗体保护剂,经口服HP1188-Ig Y可抑制小鼠胃内H.pylori感染。AIM：To evaluate the effects of treatment with HP 1188-immunoglobulin yolk （ HP1188-IgY） on Helicobacter pylori （ H.pylori）-infected gastritis in BALB/c mice.METHODS：BALB/c mice were used to establish an animal model of H.pylori-infected gastritis, and the mice were divided into 8 groups （10 mice per group）.Oral antibiotics were used in group 1, 1 mg HP1188-IgY in group 2, 1 mg HP1188-IgY plus 30%sucralfate in group 3, 5 mg HP1188-IgY in group 4, 5 mg HP1188-IgY plus 30%sucralfate in group 5, PBS in group 6, and 30% sucralfate in group 7 with the treatment once per day for 10 d;and 2.5 mg HP1188-IgY was injected hypodermically twice with a 48-h interval in group 8.Another 10 mice were used as normal control in group 9.The planting of bacteria in the stomach was assayed by bacteri-al culture, rapid urease test, PCR and pathological sectioning .RESULTS：Intragastric administration with 1 mg HP1188-IgY plus 30%sucralfate per day effectively cured the injury of gastric mucosa caused by H.pylori infection, and the effect has no significant difference compared with antibiotics （P〉0.05）.CONCLUSION：We establish a BALB/c mouse mod-el infected with H.pylori successfully.Sucralfate （30%） is an ideal protectant for HP1188-IgY, which might decrease H. pylori infection in the stomach of BALB/c mice by oral inoculation .

关 键 词：幽门螺杆菌 HP1188蛋黄抗体 硫糖铝 

分 类 号：R378.99[医药卫生—病原生物学]