人胃癌及癌旁组织RNA氧化损伤水平的比较及临床意义  

Level and clinical significance of RNA oxidative damage in human gastric cancer and para-carcinoma tissues

作　　者：林宪慧 余俊华[1] 林海鸿[1] 屠洋洋 罗顺斌[2] 徐涛[2] 陈统[1] 林型城 叶孙志 郑志强[1] 蔡剑平[2] 

机构地区：[1]温州医科大学附属第二医院,浙江温州325027 [2]卫生部北京医院,卫生部北京老年医学研究所,卫生部老年医学重点实验室,北京100730

出　　处：《中国病理生理杂志》2015年第1期172-176,共5页Chinese Journal of Pathophysiology

摘　　要：目的:通过检测人胃癌组织及其癌旁组织中的RNA氧化损伤程度,探讨RNA氧化与胃癌发生的相关性。方法:分别利用免疫组化方法和液相-串联质谱(LC-MS/MS)方法对61例胃癌组织及其癌旁组织中8-氧鸟苷(8-oxo Gsn)进行定性和定量分析,并统计分析结果。结果:免疫组化结果检测显示8-oxo Gsn在癌旁组织中含量低,而在胃癌组织中含量明显增高,且棕色染色区域主要集中在肿瘤细胞胞浆位置。质谱检测结果显示胃癌组织中8-oxo Gsn含量较对应癌旁组织高,差异有统计学意义(P<0.05)。结论:8-oxo Gsn在胃癌组织中含量增加。RNA氧化损伤可能在胃癌的发生发展过程中起重要作用。AIM：To investigate the RNA oxidative damage in human gastric cancer tissue and para-carcinoma tissue for exploring the role of RNA oxidation in the occurrence of gastric cancer .METHODS：Immunohistochemical ob-servation and LC-MS/MS analysis were performed in 61 cases of gastric carcinoma .The position and concentration of 8-oxoguanosine （ 8-oxoGsn ） were detected , respectively . RESULTS： The results of immunohistochemical observation showed that 8-oxoGsn was obviously up-regulated in the gastric cancer .The positive staining mainly accumulated in the cy-toplasm of the tumor cells .The results of mass spectrometry showed that the level of 8-oxoGsn in the gastric cancer tissues was higher than that in the para-carcinoma tissues （P〈0.05）.CONCLUSION：8-oxoGsn is up-regulated in gastric canc-er.RNA oxidative damage may play important roles in the occurrence of gastric cancer .

关 键 词：胃肿瘤 免疫组织化学 8-氧鸟苷 液相-串联质谱 

分 类 号：R735.2[医药卫生—肿瘤]