长白山濒危树种刺楸组培快繁技术研究  

Study on The Rapid Micropropagation Technology in Kalopanax septemlobus of Changbaishan

作　　者：王有菊[1] 高扬[1] 王继志[1] 迟锐[1] 

机构地区：[1]吉林市林业科学研究院,吉林吉林132013

出　　处：《林业科技》2015年第1期1-5,共5页Forestry Science & Technology

基　　金：吉林省林业厅"林业科技项目"资助(2012-003)

摘　　要：以长白山刺楸人工林选择的刺楸优良个体休眠枝条为试验材料,进行刺楸组培快繁各个阶段最适培养基的筛选。结果表明,刺楸组织培养的最适外置体为叶柄;愈伤组织诱导的最适培养基为WPM+2,4-D0.50mg/L+NAA0.1 mg/L,诱导率96%;愈伤组织诱导再分化最适培养基为WPM+KT2.0 mg/L+6-BA0.50 mg/L+NAA 0.1 mg/L,分化率达82%;不定芽继代培养最适培养基为WPM+KT1.0 mg/L+6-BA0.50 mg/L+NAA0.1 mg/L,增殖系数为3.69;不定芽生根培养最适培养基为WPM+NAA 0.5 mg/L,生根率达到85%。The dormant wood of Kalopanax septemlobus in Changbaishan were used as experimental materials .The experiment was made to find out the best tissue culture process and culture medium .The results showed that the leafstalk was the suitable explant and tissue culture of Kalopanax septemlobus required different kinds of culture medium in different phases .The optimal initiation medium is WPM ＋2, 4-D0.50 mg/L ＋NAA0.1 mg/L, the rate of iniation is over 90%; The callus redifferentiation medium is WPM ＋KT2.0 mg/L＋6 -BA0.50 mg/L＋NAA0.1 mg/L, the rate of redifferectiation is 82%; The proper rooting medium is WPM ＋NAA 0.5 mg/L, and the rooting rate can be up to 85%.

关 键 词：刺楸 组培快繁 愈伤组织再生体系 

分 类 号：S792.21[农业科学—林木遗传育种] S722.37[农业科学—林学]