Chemical compositions and effects on chemiluminescence of AMs in vitro of chalk dusts  

作　　者：Yue-Xia Zhang Zhen-Hua Yang Quan-Xi Zhang Rui-Jin Li Hong Geng Chuan Dong 

机构地区：[1]Institute of Environmental Science,Shanxi University

出　　处：《Chinese Chemical Letters》2015年第1期157-159,共3页中国化学快报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(Nos.21107064,2140509,21175086 and 21177078);by grant from Shanxi Scholarship Council of China(Nos.2013-012 and 2013-016)

摘　　要：The aim of this study was to investigate the chemical compositions of chalk dust and examine the adverse effects of fine chalk particle matters（PM（2.5）） on rat alveolar macrophages（AMs） in vitro.Morphologies and element concentrations of chalk particles were analyzed using quantitative energydispersive electron probe X-ray microanalysis（ED-EPMA）.The oxidative response of AMs exposed to chalk PM（2.5） was measured by luminol-dependent chemiluminescence（CL）.The results showed that（1）Chalk dust was mainly composed of gypsum（CaSO4）,calcite（CaCO3）/dolomite（CaMg（CO3）2）,and organic adhesives;（2） Fine chalk particles induced the AM production of CL,which was inhibited by about 90%by diphenyleneiodonium chloride（DPI）.Based on these results,we showed that cytotoxicity of chalk PM（2.5） may be related to the reactive oxygen species（ROS） generation.The aim of this study was to investigate the chemical compositions of chalk dust and examine the adverse effects of fine chalk particle matters（PM（2.5）） on rat alveolar macrophages（AMs） in vitro.Morphologies and element concentrations of chalk particles were analyzed using quantitative energydispersive electron probe X-ray microanalysis（ED-EPMA）.The oxidative response of AMs exposed to chalk PM（2.5） was measured by luminol-dependent chemiluminescence（CL）.The results showed that（1）Chalk dust was mainly composed of gypsum（CaSO4）,calcite（CaCO3）/dolomite（CaMg（CO3）2）,and organic adhesives;（2） Fine chalk particles induced the AM production of CL,which was inhibited by about 90%by diphenyleneiodonium chloride（DPI）.Based on these results,we showed that cytotoxicity of chalk PM（2.5） may be related to the reactive oxygen species（ROS） generation.

关 键 词：CHEMILUMINESCENCE Chalk PM2.5 Alveolar macrophages Reactive oxygen species 

分 类 号：R135.2[医药卫生—劳动卫生]