罗汉果实时荧光定量PCR内参基因的选择  

Selection of reference genes of Siraitia grosvenorii by real-time PCR

作　　者：涂冬萍[1] 莫长明[2] 马小军[1] 赵欢[1] 唐其[3] 黄杰[4] 潘丽梅[2] 韦荣昌[2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]广西药用植物园,广西南宁530023 [3]湖南农业大学园艺园林学院,湖南长沙410128 [4]广西中医药大学,广西南宁530001

出　　处：《中国中药杂志》2015年第2期204-209,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81373914;31400275);国家"十二五"科技支撑计划项目(2011BAI01B03);广西自然科学基金项目(2012GXNSFAA053043)

摘　　要：罗汉果为药食两用的传统中药,研究从罗汉果转录组中选取了Actin,18SrRNA,ubiquilin,ef1-α,ef1-β,Tubulin作为候选基因,通过实时荧光定量PCR,利用ge Norm,Norm Finder,Best Keeper,Delta CT,Ref Finder软件和方法对6个候选参考基因在不同罗汉果样品中的表达稳定性进行分析,首次筛选出了不同时期的有籽和无籽罗汉果果实中较理想的内参基因,结果发现18SrRNA在所有罗汉果样品中表达最稳定,是样品基因分析适合的内参基因,对采用qRT-PCR方法分析罗汉果基因表达中内参基因的选择具有指导作用,也为罗汉果生物合成途径基因及其他不同条件下基因差异表达研究提供参考,确保罗汉果基因表达分析结果的可靠性。Siraitia grosvenorii is a traditional Chinese medicine also as edible food. This study selected six candidate reference genes by real-time quantitative PCR, the expression stability of the candidate reference genes in the different samples was analyzed by using the software and methods of geNorm, NormFinder, BestKeeper, Delta CT method and RefFinder, reference genes for S. grosve- norii were selected for the first time. The results showed that 18SrRNA expressed most stable in all samples, was the best reference gene in the genetic analysis. The study has a guiding role for the analysis of gene expression using qRT-PCR methods, providing a suit- able reference genes to ensure the results in the study on differential expressed gene in synthesis and biological pathways, also other genes of S. grosvenorii.

关 键 词：罗汉果 内参基因 实时定量PCR 

分 类 号：S567.239[农业科学—中草药栽培]