砂炒对波棱瓜子有效成分、致腹泻以及保肝作用的影响  

Influence of stir-baked with sand on active ingredients,diarrhea and hepatoprotection of Herpetospermum caudigerum

作　　者：李娟娟[1,2] 沈刚[1,2] 尹蓉莉[1] 沈成英[1,2] 程玲[1,2] 邱玲[1,2] 韩晋[2] 袁海龙[2] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]中国人民解放军第三〇二医院,北京100039

出　　处：《中国中药杂志》2015年第2期236-239,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2015ZX09101025);北京市科委重点项目(Z14110700220000)

摘　　要：为了研究砂炒对波棱瓜子有效成分、致腹泻以及保肝作用的影响,该实验采用HPLC分析波棱瓜子砂炒前后有效成分波棱甲素与波棱素的含量差异;以稀便率和腹泻指数评价砂炒对波棱瓜子总木脂素(total lignans,TL)致泻情况的影响;以CCl4急性肝损伤模型评价砂炒对波棱瓜子TL保肝作用的影响。HPLC分析结果显示,波棱瓜子砂炒后波棱甲素含量降低40.9%(P<0.01),波棱素含量无显著性变化。药效学结果显示,砂炒波棱瓜子TL高、中剂量组稀便率与腹泻指数显著低于同剂量生品波棱瓜子TL组(P<0.01或P<0.05);砂炒及生品波棱瓜子TL高、中剂量组均能显著抑制CCl4致肝损伤小鼠血清中ALT,AST水平的升高(P<0.01或P<0.05),但砂炒波棱瓜子TL高、中剂量组小鼠ALT,AST水平高于同剂量的生品波棱瓜子TL组(P<0.05)。结果表明,砂炒可有效缓解波棱瓜子TL致泻作用,但是同时也降低了波棱瓜子TL的保肝作用。To study the influence of stir-baked with sand on active ingredients, diarrhea and hepatoprotection of Herpetospermum caudigerum, the contents of herperione and herpetin in H. caudigerum before and after stir-baking with sand were analyzed by HPLC. The effect of stir-baked with sand on diarrhea of H. caudigerum TL was evaluated using the mean stool rate （MSR） and mean diarrheal index （MDI） and the influence of stir-baked with sand on hepatoprotective effect of H. caudigerum TL was examined using a mouse model of CCl4-induced liver injury based on the analysis of serum ALT and AST activities. The results of HPLC analysis showed the content of herperione in H. caudigerum after stir-baking with sand decreased by 40. 9% （P 〈 0. 01 ） and the content of herpetin had no change. Pharmacodynamic results showed that the MSR and MDI of high-dose and middle-dose group of H. caudigerum TL after stir- baking with sand were significantly lower than that of high-dose and middle-dose group of H. caudigerum TL without stir-baking with sand ; The high-dose and middle-dose of H. caudigerum TL with/without stir-baking with sand significantly alleviated liver injury as in- dicated by the decreased levels of serum ALT and AST, but the ALT and AST levels of high-dose and middle-dose group of H. caudi- gerum TL after stir-baking with sand were higher than that of H. caudigerum TL without stir-baking with sand. The results revealed that the stir-baking with sand could effectively relieve diarrhea effect of H. caudigerum TL, while it also reduces the hepatoprotection of H. caudigerum TL.

关 键 词：波棱瓜子 总木脂素 砂炒 腹泻 保肝作用 

分 类 号：R285[医药卫生—中药学]