补阳还五汤诱导脑缺血后血管生成促进侧脑室下区神经母细胞迁移  

Buyang Huanwu decoction promotes neuroblast migration from subventricular zone via inducing angiogenesis after ischemia

作　　者：李琳[1] 刘志婷[2] 储利胜[2] 俞天虹[2] 曲铁兵[2] 王俊[1] 任翠翠[1] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江中医药大学基础医学院,浙江杭州310053

出　　处：《中国中药杂志》2015年第2期298-302,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81073075;81274113);浙江省自然科学基金项目(Y207771)

摘　　要：目的:研究补阳还五汤诱导脑缺血后血管生成对侧脑室下区神经母细胞迁移的影响及机制。方法:采用线栓法诱导小鼠大脑中动脉阻塞模型,缺血30 min,分假手术组、模型组、补阳还五汤组和内皮抑素组。补阳还五汤组缺血后24 h开始灌胃补阳还五汤(20 g·kg-1),内皮抑素组在灌胃补阳还五汤同时皮下注射内皮抑素(10μg/只),每天1次,连续14 d。缺血后第14天,采用免疫荧光法检测缺血周边区微血管密度和神经母细胞数量,采用实时荧光定量PCR和Western blot检测基质细胞衍生因子1(stromal cell-derived factor-1,SDF-1)、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)mRNA和蛋白表达。结果:与模型组比较,补阳还五汤组缺血周边区微血管密度和神经母细胞数量显著增多(P<0.01),SDF-1和BDNF mRNA和蛋白表达增加(P<0.01);与补阳还五汤组比较,内皮抑素组小鼠缺血周边区微血管密度和神经母细胞数量显著减少(P<0.01),SDF-1和BDNF mRNA和蛋白表达显著下降(P<0.01)。结论:补阳还五汤促进脑缺血后血管生成有助于侧脑室下区神经母细胞向缺血周边区迁移,机制可能与上调SDF-1和BDNF表达有关。Objective： To study the effect of Buyang Huanwu decoction （BYHWD） inducing angiogenesis on the neuroblast migra- tion from the subventricular zone and its mechanisms after focal cerebral ischemia. Method： The middle cerebral artery occlusion （MCAO） was performed to mice for 30 minutes to establish the model. The rats were divided into sham group, model group, BYHWD group and endostatin group. BYHWD （20 g · kg ^-1, ig） and endostatin （10 μg, sc） were administered 24 h after ischenmia once a day for consecutively 14 days. At 14 d after ischemia, the density of micro-vessel and the number of neuroblasts in the isehemia border zone were determined by immunofluorescence staining. The mRNA and protein expression of cell-derived factor-1 （ SDF-1 ） and brain- derived neurotrophic （BDNF） were examined by real-time PCR and Western blot. Result： Compared with the model group, BYHWD significantly increased the density of micro-vessel and the number of DCX positive cells in the ischemia border zone （ P 〈 0.01 ） , and significantly increased the SDF-1 and BDNF mRNA and protein expression （P 〈 0.01 ）. Compared with BYHWD group, endostatin significantly reduced the density of micro-vessel and the number of DCX positive cells in the ischemia border zone （ P 〈 O. 01 ） , as well as the SDF-1, BDNF mRNA and protein expression （P 〈 0.01 ）. Conclusion： BYHWD could promote the neuroblast migration from the subventricular zone via inducing angiogenesis after cerebral ischemia, the mechanism may be correlated with up-regulating the ex- pression of SDF-1 and BDNF.

关 键 词：补阳还五汤 局灶性脑缺血 血管生成 神经母细胞 迁移 

分 类 号：R285.5[医药卫生—中药学]