雪松不同部位中莽草酸的含量分析  

Analysis on the Content of Shikimic Acid in Different Parts of Cedrus deodara

作　　者：雷艳萍[1,2] 石晓峰[1] 白朝辉[2] 刘东彦[1] 李师[2] 

机构地区：[1]甘肃省医学科学研究院药物研究所,兰州730050 [2]甘肃省高校中(藏)药化学与质量研究省级重点实验室/甘肃中医学院,兰州730030

出　　处：《中国药房》2015年第6期803-805,共3页China Pharmacy

基　　金：甘肃省科技支撑计划项目(No.1204FKCA152);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(No.zzy-2011-05)

摘　　要：目的：测定雪松不同部位的莽草酸含量,为进一步开发利用雪松提供参考。方法：采用高效液相色谱法。色谱柱为Diamonsil C18,流动相为0.1%磷酸水溶液-甲醇（95∶5,V/V）,流速为1.0 ml/min,检测波长为217 nm,柱温为30℃,进样量为20μl。结果：莽草酸的质量浓度在1～50μg/ml范围内与其峰面积呈良好的线性关系（r=0.999 8）;精密度试验的RSD〈2%,重复性、稳定性试验的RSD〈3%;平均加样回收率为99.2%,RSD=2.08%（n=9）。雪松不同部位中莽草酸的含量依次为松针〉嫩枝〉花序〉老枝〉老枝的树皮〉老技的木质部,其中以雪松松针最高,达3.22%。结论：雪松的松针和嫩枝所含的莽草酸较高,具有较高的开发价值。OBJECTIVE： To determine the content of shikimic acid in different parts of Cedrus deodara and provide reference for its further development and application. METHODS： HPLC was conducted with Diamonsil C18 column, 0.1% Phosphoric ac- id-methanol solution （95：5, V/V） mobile phase at a flow rate of 1.0 ml/min. The detection wavelength was 217 nm, column temper- ature was 30 ℃ and injection volume was 20 μl. RESULTS： The concentration of shikimic acid had good linear relationship with its peak area values in the range of 1-50 μg/ml（r=0.999 8）. The RSD of precision test was less than 2% and the RSDs of repeat- ability and stability test were less than 3%; the average sample recovery were 99.2%, the RSD were 2.08% （n=9）. The content of shikimic acid from different part of C. deodara was as follows： the pine needles〉tender branches〉inflorescence〉old branches〉 tree bark of old branches〉xylem of old branches, in which the C. deodara pine needles stand top, accounting for 3.22%. The con- tent of shikimic acid in tree bark from old branches of C. deodara is 1.67%, which is more than that of wood. CONCLUSIONS： The content of shikimic acid in the pine needles and tender branches of C. deodara stand the highest with high development value.

关 键 词：雪松 不同部位 莽草酸 含量分析 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]