三七总皂苷对大鼠缺血心肌血管新生及相关生长因子的影响  

Impact of PNS on Angiogenesis in Ischemic Myocardium of Rats and Related Growth Factors

作　　者：陈社带[1] 邹燕[2] 赵伟国[1] 刘锐锋[1] 李运景[1] 

机构地区：[1]中山市人民医院药学部,广东中山528403 [2]广东药学院附属第二医院检验科,广东广州510300

出　　处：《辽宁医学院学报》2015年第1期7-9,共3页Journal of Liaoning Medical University (LNMU) Bimonthly

基　　金：中山市卫生和计划生育局医学科研立项课题;编号:2014J061

摘　　要：目的观察三七总皂苷促对大鼠缺血心肌血管新生及相关生长因子表达的影响。方法结扎左冠状动脉制备急性心肌梗死(AMI)模型大鼠,随机分为模型组和三七总皂苷低、高剂量组,另设假手术组。比较各组大鼠心肌微血管密度(MVD)和血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(b FGF)蛋白表达灰度值。结果模型组和三七总皂苷组大鼠MI边缘区MVD及VEGF、b FGF蛋白表达灰度值均明显高于假手术组(P<0.01);三七总皂苷组大鼠MI边缘区MVD及VEGF、b FGF蛋白表达灰度值明显高于模型组(P<0.05或P<0.01)。结论三七总皂苷可促进心肌梗死(MI)后大鼠缺血心肌血管新生而发挥缺血心肌保护作用,可能机制为增强心肌VEGF、b FGF蛋白表达。Objective To observe the effect of PNS in promoting angiogenesis in ischemic myocardium of rats and related growth factor expression. Method The left coronary artery of model rates with acute myocardial infarction（ AMI） was ligated. The rats were randomly divided into model group,groups of low and high dose of PNS,and sham operation group. The myocardial micro- vascular density（ MVD）,vascular endothelial growth factor（ VEGF）,and protein expression gray value of basic fibroblast growth factor（ b FGF） in rats of each group were compared. Results The MVD,VEGF and protein expression gray values of b FGF within MI marginal zone of rats in model group and PNS group were significantly higher than that of sham operation group（ P〈0. 01）; The M VD,VEGF and protein expression gray values of b FGF within MI marginal zone of rats in PNS group were significantly higher than that of model group（ P〈0. 05 or P〈0. 01）. Conclusion PNS can promote angiogenesis in ischemic myocardium of rats after myocardial infarction（ MI）,play a protective role in myocardial angiogenesis and may enhance myocardial VEGF and b FGF protein expression for the mechanism.

关 键 词：三七总皂苷 心肌梗死 血管新生 生长因子 

分 类 号：Q954.56[生物学—动物学]