柱前衍生化HPLC测定石榴籽油中石榴酸的含量  

Determination of Punicic Acid in Pomegranate Seed Oil by Precolumn Derivatization HPLC

作　　者：李艳[1] 高英[1] 辛永涛[1] 张静[1] 李卫民[1] 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2015年第4期68-70,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：石榴酸（C18：3）在结构上为顺9，反11，顺13-十八碳三烯酸，为长链烃类，在200-400nm没有吸收，无法直接用紫外检测器进行检测，本实验采用柱前衍生化方法，使石榴酸带上一个强发色团，使其在200-400nm有最大吸收，建立石榴籽油中指标性成分石榴酸的柱前衍生化HPLC分析方法。方法：以ω-溴代苯乙酮作为衍生化试剂，以三乙醇眩为相转移催化剂，对石榴籽油进行柱前衍生化，色谱条件采用Diamonsi C18色谱柱（4.6mm×250mm，5μm），检测波长272nm，以甲醇-乙腈-水（68.5：20：11.5）为流动相，柱温30℃，流速1.0mL·min^-1。结果：石榴酸在0.0266-0.1330g·L^-1（r=0.999）线件关系良好，平均加样回收率为98.7％，RSD1.8％。结论：该法稳定性、重复性好，定量准确，可作为石榴籽油中“榴酸的定量方法，用于评价和控制石榴籽油的质量。Objective： As the long chain hydrocarbons, punicic acid （C18：3） on the structure of 9 （Z） , 11 （E）, 13 （Z） -octadecatrienoic acid, which was in 200-400 nm absorption-free and can＇t be measured directly by UV detector. This experiment adopted column derivatization methods, made the punicic acid with a strong ehromophore in maximum absorption in 200-400nm. The determination method of punicicacid in pomegranate seed oil by HPLC was established in this thesis. Method： The punicic acid were derivatized with ω-bromoacetophenone as derivative reagent, triethanolamine as catalyst, the Diamonsi Clseolumn （4.6 mm×250 mm, 5μm） was used. The detection wavelength was set at 272 nm, column temperature 30℃, with methanol- acetonitrile-water （68.5： 20：11.5） aseluent, the flow rate was 1.0 mL·min^-1 Result ： The standard curves of punicic acid is linear within in the range of 0.0266-0.1330g·L^-1 and the coefficient is 0.999. The average recovery of punieie acid is 98.7% , RSD is 1.8%. Conclusion： The method is reproducible and accurate, which can be used as the determination method of punicic acid inpomegranate seed oil, and used for the quality control of pomegranate seed oil.

关 键 词：石榴籽油 石榴酸 柱前衍生化 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]