补肾固本方对去卵巢大鼠骨质疏松症骨密度、骨矿盐含量及CT含量的影响  

Effect of Bushen Guben Decoction on Bone Mineral Density,Bone Mineral Content and Calcitonin Level in Ovariectomized Rat Model of Osteoporosis

作　　者：黄继锋[1] 宋敏[1] 李泽佳[1] 刘彦宏[1] 

机构地区：[1]甘肃中医学院,兰州730000

出　　处：《中国实验方剂学杂志》2015年第4期149-152,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：甘肃省财政厅基本科研业务费专项基金项目(BH2010-018);甘肃省自然科学基金项目(2009-GS03239)

摘　　要：目的:采用切除双侧卵巢复制骨质疏松症大鼠模型,观察补肾固本方对去势大鼠骨质疏松症骨密度(BMD)、骨矿盐含量及血清降钙素(CT)水平的影响。方法:将105只6月龄雌性Wistar大鼠,随机分为空白组、模型组、仙灵骨葆组(500mg·kg-1)、戊酸雌二醇组(90μg·kg-1)、补肾固本方高、中、低剂量组(16,8,4 g·kg-1)。切除双侧卵巢复制骨质疏松症大鼠模型,造模成功后,给药组ig给予相应药物,给药3个月后取材。双能X射线BMD测量仪及X射线分别测量股骨和腰椎BMD及骨矿盐含量,补肾固本方对股骨及腰椎骨生物力学性能的作用,ELISA法检测CT。结果:与正常组比较,模型组股骨BMD值及骨矿物含量有明显下降(P<0.05),模型组大鼠股骨及腰椎的弹性模量、最大载荷、屈服强度下降(P<0.05),去卵巢组大鼠的血清CT水平明显升高(P<0.01);与模型组比较,各治疗组BMD及骨矿物有所提高(P<0.05,P<0.01),补肾固本方高、中、低剂量组及仙灵骨葆组BMD显著提高(P<0.01),补肾固本方高、中剂量对大鼠腰椎生物力学有一定改善(P<0.05),高剂量组对腰椎弹性模量有明显改善(P<0.01),补肾固本方高、中、低剂量组CT水平较模型组明显改善(P<0.05),其中补肾固本方高剂量组大鼠血清CT水平均明显高于其余各组,中剂量组对CT水平有明显改善(P<0.01),高、中剂量组间比较无差别。结论:补肾固本方能明显改善去卵巢大鼠BMD、骨矿物含量、改善去卵巢大鼠血CT水平,BMD及骨矿物含量明显降低;骨的力学性能得到改善或恢复。Objective： To observe the effect of Bushen Guben decoction （BGD） on bone mineral density （BMD）, bone mineral content and serum calcitonin （CT） level in an ovariectomized rat model of osteoporosis. Method： One hundred and five female Wistar rats were randomly divided into the control group, the model group, the Xianlin Gubao group （500 mg·kg^-1）, the estradiol valerate group （90μg·kg^-1） and high-, medium-, low-dose BGD groups （16, 8, 4 g·kg^-1）. The rats except the control group were subjected to bilateral ovariectomy and killed after 3-month treatment. BMD and bone mineral content in the femur and lumbar vertebra, their biomechanical properties were measured by dual-energy X-ray absorptiometry measurement instrument and X-ray. The serum CT level was detected by ELISA method. Result： Compared with the normal group, the BMD and bone mineral content decreased significantly （P 〈 0. 05 ）, elasticity modulus, maximum loads and yield strength of femur and lumbar vertebra reduced （P 〈 0.05 ）, serum CT level declined （P 〈 0.01 ） in the model group. Compared with the model group, the BMD and bone mineral content increased in all treatment groups （P 〈 0. I）5, P 〈 0.01 ）, the biomechanical properties had good improvement in Xiaoling Gubao group and high-, medium-dose BGD groups （P 〈0.05, P 〈0.01 ） , serum CT level raised in high-, medium-, low-dose BGD groups （ P 〈 0.05, P 〈 0.01 ）. High-dose BGD had better results in the elasticity modulus of lumbar vertebra and CT level than other groups. Conclusion： BGD had good improvement on osteoporosis in ovariectomized rats by affecting BMD and bone mineral content, CT level and biomechanical properties.

关 键 词：补肾固本方 骨质疏松症 骨密度 骨矿盐 血清降钙素含量 

分 类 号：R285.5[医药卫生—中药学]