γ-干扰素受体在山羊颈前神经节的表达  

Expression of IFNGR in the Cranial Cervical Ganglion of Goat

作　　者：李强[1] 王志豪[1] 金秀芳[1] 徐永平[1] 陈文东[1] 黄双[1] 孙曼[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农业学报》2015年第1期7-12,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31072184);陕西省农业推广项目(k332021309)

摘　　要：旨在探索山羊颈前神经节是否具备接受γ-干扰素(interferon-γ,IFN-γ)作用的条件,是否有IFN-γ受体(interferon-γreceptor,IFNGR)的存在,进而明确神经调节与免疫调节之间的关系。分别取雌雄成年山羊颈前神经节各5对,用免疫组化SP法和PCR方法检测IFNGR在颈前神经节的表达情况。结果表明,在山羊颈前神经节的神经元、卫星细胞和神经纤维均有IFNGR免疫阳性产物分布,但主要分布于神经元胞体,IFNGR在神经元胞体的相对表达量显著高于非神经细胞结构(P<0.05)。在山羊颈前神经节扩增到IFNGR1基因的cDNA片段全长376bp,与绵羊、牛、野猪、家兔、褐家鼠IFNGR1基因同源性分别为98%、97%、84%、73%、66%。在山羊颈前神经节中IFNGR主要在交感节后神经元表达与分布,具备对IFN-γ刺激做出反应的条件,提示山羊颈前神经节可能作为IFN-γ对靶器官免疫内分泌调节途径和自主神经对靶器官调节的神经途径之间相互协调的关键点。This experiment was conducted to detect the existence of IFNGR in the cranial cervical ganglion（CCG）in goat,and to research the relationship between nerve regulation and immune regulation.Five pairs of CCG were taken from mature female and male goats,respectively.The expression of IFNGR was detected by PCR method and immunohistochemical SP staining.The results showed that：IFNGR immunoreactive substances were distributed in neurons,satellite cells and passing fiber,and mainly in the neurons.The relative expression of IFNGR was significantly higher than that of non-neuronal cells（P〈0.05）.The full length of IFNGR1 gene amplified by PCR method was 376 bp,and IFNGR1 gene of goat homology with sheep（98%）was the highest,followed by cattle（97%）,Sus scrofa（84%）,Oryctolagus cuniculus（73%）and Rattus norvegicus（66%）.The results suggested that the IFNGR in CCG of goat mainly expressed and located in sympathetic postganglionic neurons which were provided with the conditions for the role of IFN-γ,which implied that CCG may act as the critical point to coordinate the immune endocrine regulation of IFN-γand neuroregulation of autonomic nerve on target organ.

关 键 词：γ-干扰素受体 颈前神经节 免疫组化SP法 PCR 山羊 

分 类 号：S827[农业科学—畜牧学]