黑皮果蔗(Badila)节间蔗糖质量分数及其关键酶活性和相关基因表达的分析  

Mass Fraction of Sucrose and Activity of Key Enzymes and Related Genes Expression in Internodes of Chewing Cane(Badila)

作　　者：黄诚梅[1] 曹辉庆[1] 魏源文[1] 邓智年[1,2] 潘有强[1] 李杨瑞[1,2] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,南宁530007 [2]中国农业科学院甘蔗研究中心/农业部广西甘蔗生物技术与遗传改良重点实验室,南宁530007

出　　处：《西北农业学报》2015年第1期119-122,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金："十二五"农村领域国家科技计划(2013AA102604-1);广西自然科学基金项目:创新研究团队项目(2011GXNSFF018002);面上项目(2011GXNSFA018111);广西科学研究与技术开发计划项目:重点实验室建设项目(12-071-09);科技攻关计划(桂科攻1222009-1B);广西农业科学院基本科研业务专项(桂农科2012YT04;200802Z(基));科技发展基金(2007024)

摘　　要：以成熟期黑皮果蔗(Badila)的第2、5、8、12节间为材料,分析测定其节间蔗糖质量分数与相关关键酶如蔗糖磷酸合成酶(sucrose-phosphate synthase,SPS)、可溶性酸性转化酶(soluble acid invertase,SAI)、中性转化酶(neutral invertase,NI)活性,并采用RT-PCR对SPS、SAI基因在4个不同节间部位中的表达差异进行半定量分析。结果表明,成熟期黑皮果蔗蔗茎中不同节间的蔗糖、还原糖质量分数均随着节间的成熟而增加,成熟节间>未成熟节间;蛋白质质量分数则相反,为未成熟节间高于成熟节间。SPS活性与SPSⅢ基因表达则是在幼嫩茎的活性高于成熟茎;SAI活性也是幼嫩茎大于成熟茎,其基因表达差异表现不明显。NI活性以第5节间最低,其他3个节间无极显著差异。The 2nd,5th,8th and 12 th internode of chewing cane（Badila）was used as experimental material to assay the sucrose content and the activity of the key enzymes,i.e.,sucrose-phosphate synthase（SPS）,soluble acid invertase（SAI）and neutral invertase（NI）related to sucrose metabolism,and the expressions of genes was also assayed by semi-quantitative RT-PCR at mature stage.The results showed that the contents of sucrose and hexose sugars in different internodes increased with the numbers of internodes,and mature internodes〉 immature internodes;the soluble protein content was contrary and immature internodes 〉mature internodes.The activity of SPS enzyme and the expression of SPSⅢ gene in immature internodes were higher than of mature internodes;the activity of SAI enzyme as the same,but the gene expression had no difference.The activity of NI in No.5was lowest,and there had no difference for other 3internodes.

关 键 词：黑皮果蔗 蔗糖质量分数 蔗糖代谢相关酶 

分 类 号：S566.1[农业科学—作物学]