急性主动脉夹层患者主动脉组织和血浆中微小核糖核酸的差异表达  

Differential Expression of micro RNAs in Aortic Tissue and Plasma in Patients With Acute Aortic Dissection

作　　者：王晓建[1] 黄毕[2] 樊晓寒[3] 苏文君[4] 张良[4] 路天怡[4] 田力[2] 杨艳敏[2] 惠汝太 张澍[3] 

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心阜外心血管病医院心血管疾病国家重点实验室,北京市100037 [2]中国医学科学院北京协和医学院国家心血管病中心阜外心血管病医院急重症中心,北京市100037 [3]中国医学科学院北京协和医学院国家心血管病中心阜外心血管病医院心律失常中心,北京市100037 [4]中国医学科学院北京协和医学院国家心血管病中心阜外心血管病医院心外科,北京市100037

出　　处：《中国循环杂志》2015年第2期154-158,共5页Chinese Circulation Journal

基　　金：国家自然科学基金(81170286;81300184)

摘　　要：目的:本研究旨在寻找新的可作为急性主动脉夹层(AAD)诊断标志物的微小核糖核酸(mi RNAs)。方法:入选4例A型AAD患者的升主动脉病变组织标本(AAD1组)和4例无心血管疾病的器官捐赠者的升主动脉组织标本(NC1组);入选20例A型AAD患者(AAD2组)和20例无心血管疾病正常对照者(NC2组)并分别采集血浆,两组病例对照的年龄、性别严格匹配。利用mi RNA芯片技术,检测四组受试者的升主动脉组织和血浆mi RNAs表达谱,整合两组分析结果,确定AAD患者组织和血浆均差异表达的mi RNAs。结果:主动脉组织miR NA芯片结果显示,AAD患者中30个miR NAs表达显著改变,其中13个表达上调,17个表达下调。血浆miR NA芯片分析显示,AAD患者有93个miR NA差异表达,其中33个表达上调,60个表达下调。整合两个表达谱,共有4个miR NAs(miR-4313、-933、-1281和-1238)在血浆和组织均显著升高。其中,AAD1组较NC1组、AAD2组较NC2组miR-4313分别上调1.5倍和42.4倍,miR-933分别上调10.4倍和26.8倍,miR-1281分别上调1.7倍和17.8倍,miR-1238分别上调1.3倍和13.8倍。结论:通过miR NA芯片分析,我们筛选到4个在AAD患者主动脉组织和血浆中均升高的miR NAs。这些miR NA可能是诊断AAD的生物标志物,但需大样本验证。Objective： We want to identify the new microRNAs （miRNAs） biomarker for diagnosing the patients with acute aortic dissection （AAD）. Methods： Our work included 2 sets of groups.①AAD 1 tissue group, containing the aortic tissue of A type AAD patients and Control 1 group, containing the aortic tissue of the subjects without cardiovascular disease. n=4 in each group. ② AAD 2 plasma group, containing the plasma of A type AAD patients and Control 2 group, containing the plasma of the subjects without cardiovascular disease. n=20 in each group. The age and gender were strictly matched between the patients and control subjects. The miRNAs expression in ascending aortic tissue and plasma were examined by microarray analysis in 4 groups, and the results in 2 sets of groups were integrated to identify the differential expression of miRNAs in AAD patients. Results： The microarray analysis indicated that in AAD patients, the aortic tissue had 30 differentially expressed miRNAs, 13 of them were up-regulated and 17 were down-regulated;plasma had 93 differentially expressed miRNAs, 33 of them were up-regulated and 60 were down-regulated. With integrated analysis, 4 miRNAs expressions were increased in both aortic tissue and plasma as miR-4313, miR-933, miR-1281 and miR-1238. Compared with Control 1 group and Control 2 group, AAD 1 group and AAD 2 group showed up-regulated miR-4313 at 1.5-fold and 42.4-fold, up-regulated miR-933 at 10.4-fold and 26.8-fold, up-regulated miR-1281 at 1.7-fold and 17.8-fold, up-regulated miR-1238 at 1.3-fold and 13.8-fold respectively. Conclusion： There were 4 differentially expressed miRNAs in both aortic tissue and plasma in AAD patients, which might be the potential biomarkers of AAD, while large sample investigation is needed.

关 键 词：MIRNA芯片 急性主动脉夹层 miRNA差异表达 

分 类 号：R541[医药卫生—心血管疾病]