微小RNA-21-5p对大鼠高氧性急性肺损伤的影响  

Effect of microRNA-21-5p on hyperoxic acute lung injury in rats

作　　者：刘国跃[1] 陈淼[1] 戢慧 覃松[1] 梅鸿[1] 陈涛[1] 陈华军[1] 

机构地区：[1]遵义医学院附属医院重症医学科二病区,贵州遵义563300 [2]湖南省长沙市天心区第三医院,湖南长沙410001

出　　处：《中国中西医结合急救杂志》2015年第1期23-27,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：贵州省科技计划项目（2012-3152）;贵州省科学技术基金（2012-48）

摘　　要：目的：探讨微小RNA-21-5p（miR-21-5p）对大鼠高氧性急性肺损伤（HALI）的影响，为HALI的基因治疗提供理论依据。方法按随机数字表法将160只SD大鼠分为高氧对照组、磷酸盐缓冲液（PBS）组、空病毒组及miR-21-5p组4组，每组40只。高氧对照组大鼠直接置于高氧箱（氧浓度＞90%）中饲养，其余3组分别经鼻腔滴入PBS、慢病毒及miR-21-5p慢病毒各200μL，然后置于高氧箱中饲养。各组分别在高氧放置0、24、48及72 h时随机取10只大鼠进行动脉血气分析，并计算氧合指数（OI）和呼吸指数（RI）；随后颈动脉放血处死大鼠取肺组织，测量左肺湿/干质量（W/D）比值，行苏木素-伊红（HE）染色，光镜下观察右肺组织病理学改变并进行病理学评分。结果高氧损伤0 h时，各组大鼠OI、RI、肺组织病理学评分、肺W/D比值差异均无统计学意义（均P＞0.05）；随着高氧损伤时间的延长，各组OI逐渐降低，RI、肺组织病理学评分、肺W/D比值逐渐升高。与高氧对照组比较，miR-21-5p组高氧损伤24、48、72 h时OI明显增加〔mmHg（1 mmHg＝0.133 kPa）：24 h 时358.10±29.25比306.19±37.23，48 h 时336.67±29.27比269.70±29.00，72 h 时323.81±19.05比203.81±43.40，均P＜0.05〕，RI明显减少（24 h时0.23±0.05比0.31±0.06，48 h时0.28±0.07比0.38±0.06，72 h时0.30±0.04比0.46±0.07，均P＜0.05），肺组织病理学评分明显降低（分：24 h时0.60±0.52比0.90±0.74，48 h时1.30±0.95比2.90±1.20，72 h时1.90±0.88比4.70±1.57，均P＜0.05），肺W/D比值明显下降（24 h时3.77±0.38比4.14±0.46，48 h时3.83±0.31比4.56±0.34，72 h时3.89±0.31比5.32±0.27，均P＜0.05）。PBS组、空病毒组高氧损伤后各指标与高氧对照组比较差异均无统计学意义（均P＞0.05）。光镜下观察可见，随着高氧损伤时间的延长，各组大鼠肺泡结构逐渐紊乱，肺泡壁断裂破坏，肺泡间隔逐渐增宽、水肿、炎�Objective To investigate the effects of microRNA-21-5p （miR-21-5p） on hyperoxic acute lung injury （HALI） in rats and provide a theoretical basis for HALI gene therapy. Methods One hundred and sixty Sprague-Dawley （SD） rats were randomly divided into four groups with number table：hyperoxia control group, phosphate buffer saline （PBS） group, blank virus group and miRNA-21-5p group （each, n = 40）. The rats in hyperoxia control group were fed directly in the hyperoxia box （oxygen concentration 〉 90%）; in the other three groups, 200 μL PBS, 200μL slow virus and 200μL miRNA-21-5p slow virus were dropped into the nose respectively, and then they were fed in the hyperoxia box. The rats were exposed to hyperoxia in the boxes for 0, 24, 48 and 72 hours in all the groups, and at each time point, 10 rats were taken randomly from each group to perform arterial blood-gas analysis, calculate oxygenation index （OI） and respiratory index （RI）. Afterwards the rats were sacrificed by blood-letting from carotid artery under intra-peritoneal anesthesia, and the lung tissues were obtained to measure the left lung wet/dry weight （W/D） ratio, hemotoxylin-eosin （HE） staining was made and the pathological changes of the right lung were observed under light microscope and the pathological score was measured. Results At 0 hour, the OI, RI, lung W/D ratio and the lung tissue pathology score in rats with hyperoxic injury had no statistically significant differences among the four groups （all P〉0.05）. With the extension of time, the level of OI was gradually reduced, and the levels of RI, pathologic score and W/D ratio of lung tissues were gradually increased. Compared with the hyperoxia control group, in miRNA-21-5p group, the levels of OI were increased significantly at 24, 48 and 72 hours after the exposure to hyperoxia [mmHg （1 mmHg = 0.133 kPa）： 24 hours 358.10±29.25 vs. 306.19±37.23, 48 hours 336.67±29.27 vs. 269.70±29.00, 72 hours 323.81±19.05 vs. 203.81±43.40, all P 〈 0

关 键 词：肺损伤 急性 高氧性 氧合指数 呼吸指数 肺湿/干质量比值 

分 类 号：R563[医药卫生—呼吸系统]