甜瓜自毒作用相关MYB转录因子的克隆和表达分析  

Cloning and Expression Analysis of One Related to Autotoxicity MYB Transcription Factor in Melon

作　　者：奚玉培[1] 张志忠[1] 谢新蕊[1] 高强[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002

出　　处：《热带作物学报》2015年第2期296-301,共6页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划项目(No.2007BAD07B03);福建农林大学园艺学院青年学术骨干培养基金(No.FAFU2012YYPY08);福建省博士后研究经费资助

摘　　要：根据甜瓜自毒作用相关MYB转录因子核心区序列设计引物,采用RACE技术获得MYB c DNA全长。生物信息学分析表明,该转录因子全长1 164 bp,包括174 bp的5′非翻译区,105 bp的3′非翻译区,885 bp的开放阅读框,编码294个氨基酸,属于MYB类转录因子中R2R3-MYB类型,与黄瓜R2R3-MYB类转录因子的氨基酸序列的同源性达97.28%。实时荧光定量PCR结果表明,该基因在植株浸提液胁迫处理2 d时表达量最高,推测该转录因子可能参与了甜瓜自毒相关基因初期的诱导表达,使植株对自毒胁迫做出主动的系统性应答反应。The full-length cDNA of one melon MYB transcription factor was cloned by RACE technology. The primers were designed according to the core sequence of MYB transcription factor related to melon autotoxicity. Bioinformatic analysis showed that the total length of the transcription factor was 1 164 bp, encoding 294 amino acids, including a 5＇untranslated region of 174 bp and a 3＇untranslated region of 105 bp and an open reading frame of 885 bp. The MYB transcription factor belonged to the R2R3-MYB type. The homology of amino acid sequence of R2R3-MYB transcription factor was 97.28% between muskmelon and cucumber. The result of real time fluorescence quantitative PCR showed that the highest gene expression was at 2 d after plant aqueous extracts treatment. The melon R2R3-MYB transcription factor may be involved in the early induction of genes expression related autotoxicity, then the plant made a initiative systemic response to autotoxicitv stress.

关 键 词：甜瓜 自毒作用 MYB转录因子 植株浸提液 

分 类 号：S652[农业科学—果树学]