海南省象耳豆根结线虫的鉴定研究  

Identification of the Root Knot Nematode Meloidogyne enterolobii in Hainan Province

作　　者：龙海波[1] 孙艳芳[1] 白成[1] 郭建荣[1] 曾凡云[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,农业部热带作物有害生物综合治理重点实验室,海南省热带农业有害生物监测与控制重点实验室,海南海口571101

出　　处：《热带作物学报》2015年第2期371-376,共6页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.314103);中央级公益性科研院所基本科研业务费专项(No.20131J001)

摘　　要：象耳豆根结线虫寄主范围广、致病力强以及能在携带Mi抗性基因的作物上寄生繁殖,是热区最具危害性的病原根结线虫种类之一。应用比较形态学,结合mt DNA序列分析、IGS序列分析和进化发育树等分子生物学方法对海南省18个市县的根结线虫进行鉴定,确定象耳豆根结线虫在蔬菜、番石榴、哈密瓜和枣树等作物上广泛发生为害,其中枣树为首次发现的象耳豆根结线虫新寄主。研究结果表明,象耳豆根结线虫已成为海南省蔬菜作物上重要的病原根结线虫种,并有进一步扩散加重为害的趋势。Meloidogyne enterolobii is one of the most damaging root-knot nematode species in tropical regions due to its wide host range, pathogenicity and ability to develop and reproduce on several crops carrying resistance genes. In the study, we used morphological methods combined with mtDNA and IGS sequences analysis and phylogenesis for identifying the root knot nematode M. enterolobii occurring in Hainan. Based on both of morphological and molecular characteristics, we confirmed that M. enterolobii is widely distributed in most of cities and counties of Hainan, and its host crops include vegetables, hami melon, guava and jujube. The results indicated that M. enterolobii is now become a major parasite on vegetable crops in Hainan showing a tendency of further spreading.

关 键 词：象耳豆根结线虫 鉴定 会阴花纹 线粒体DNA 进化分析 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]