红霉素对4-羟基壬烯醛刺激的气道上皮细胞MMP-2、MMP-9、COX-2  

Effects of erythromycin on expression of MMP-2, MMP-9, COX-2, and AP-2 in airway epithelial cells induced by 4-hydroxy-2-nonenal

作　　者：柯明耀[1] 薛克营[1] 吴雪梅[1] 谢红旗[1] 罗炳清[1] 赵年贵[1] 

机构地区：[1]福建医科大学教学医院厦门市第二医院厦门市呼吸中心,361021

出　　处：《国际呼吸杂志》2015年第4期246-249,共4页International Journal of Respiration

基　　金：福建省卫生厅医学创新课题（2009-CXB-63）

摘　　要：目的观察红霉素对4-羟基壬烯醛（4-HNE）刺激的气道上皮细胞基质金属蛋白酶2（MMP-2）、MMP-9、环氧合酶2（COX2）和活化蛋白-2（AP-2）表达的影响。方法Westernblot检测红霉素（5μmol／L）预孵育不同时间（24、36、48h）后4HNE（10μmol／L作用4h）刺激的气道上皮细胞MMP2、MMP9、COX2和AP-2蛋白表达的变化。结果红霉素预孵育36h和48h后明显抑制4HNE刺激的气道上皮细胞MMP-2、MMP-9、COX-2合成，明显抑制4-HNE刺激的气道上皮细胞核转录因子AP2的激活，与红霉素未预孵育组相比，差异具有统计学意义（P〈0．05）。结论红霉素可明显抑制4HNE刺激的气道上皮细胞MMP2、MMP-9、COX-2表达，其机制可能和抑制核转录因子AP2的激活有关。Objective To observe the effects of erythromycin on the expression of matrix metalloproteinase-2 （MMP 2 ）, MMP-9, cyclooxygenase-2 （ COX-2 ） and activator protein-2 （ AP-2 ） in airway epithelial cells induced by 4-hydroxy 2 nonenal （4-HNE）. Methods Airway epithelial cells were preincubated with 5 μmol/L erythromycin for different time （24, 36, 48 hours） and incubated with 10 μmol/L 4-HNE for four hours. The protein expressions of MMP-2, MMP-9, COX-2, and AP 2 were detected by Western blot. Results Preincubation with 5 μmol/I. erythromyein for 36 hours and 48 hours significantly inhibited the expressions of MMP 2, MMP 9, COX2, and AP-2 in airway epithelial ceils induced by 4-HNE. Compared with the control group, the difference was significant （P〈0.05）. Conclusions Erythromycin could inhibit the expressions of MMP 2, MMP 9, and COX 2 in airway epithelial cells induced by 4 HNE,which may be associated with inhibiting the stimulation of AP-2.

关 键 词：红霉素 4-羟基壬烯醛 气道上皮细胞 基质金属蛋白酶2 基质金属蛋白酶9 环氧合酶2 活化蛋白-2 

分 类 号：R651.15[医药卫生—外科学]