NAP3在结核病诊断中的价值  

：Diagnostic value of NAP3 for tuberculosis

作　　者：曹志红 曹彦 程小星 

机构地区：[1]解放军第三.九医院全军结核病研究所全军结核病防治重点实验室结核病诊疗新技术北京市重点实验室,北京100091

出　　处：《国际呼吸杂志》2015年第4期257-260,共4页International Journal of Respiration

基　　金：国家自然科学基金资助项目（81071318）

摘　　要：目的研究肺结核患者外周血单个核细胞（PBMCs）经结核特异性抗原刺激后中性粒细胞激活蛋白3（NAP3）的表达情况并与结核潜伏感染（latent tuberculosis infection，LTBI）者及非结核感染健康对照者进行比较。方法提取6例活动期肺结核患者和6例LTB1个体PBMCs，用结核分枝杆菌特异性抗原肽刺激后收集细胞，采用基因芯片分析方法比较两组NAP3表达情况。然后提取60例活动期肺结核患者和60名PPD阳性健康个体（根据γ干扰素表达情况将其分成LTBI组和非结核感染健康对照组）的PBMCs，用结核分枝杆菌特异性抗原肽刺激后，采用酶联免疫吸附试验（ELISA）定量检测刺激前、后细胞培养液上清中NAP3的浓度。结果基因芯片分析发现活动期肺结核患者NAP3表达明显高于LTBI组（U=1．000，P=0．0043）。ELISA分析结果发现特异性抗原刺激前细胞培养液上清中3组的NAP3浓度差异无统计学意义（F=0．7341，P=0．4821），而刺激后只有活动期肺结核组和非结核感染健康对照组之间的NAP3浓度相比差异有统计学意义（P=0．002）。结论高表达的NAP3可作为诊断结核的指标之一，但是无法区分潜伏感染和活动期感染病例。Objective To compare the neutrophil activating protein 3 （NAP3） expression from peripheral blood mononuelear cells （PBMCs） stimulated with Mycobacterium tubereulosis-speeific antigens in pulmonary tuberculosis patients with latent tuberculosis infection （LTBI） and non-tuberculosis infection healthy controls. Methods The NAP3 level in PBMCs stimulated with Myeobacterium ruberculosis-speeifie antigens from six active tuberculosis patients and six LTBI controls was compared using microarray analysis method. Then the concentration of NAP3 in culture supernatants of PBMCs stimulated with Mycobacterium tuberculosis-specific antigens from 60 active tuberculosis patients and 60 PPD-positive healthy controls （divided into LTBI group and non-tuberculous infection healthy control group based on the expression of interferow7 ） was quantitatively detected by enzyme-linked immunosorbent assay （ELISA）. Results Microarray analysis showed that NAP3 level in patients with active tuberculosis was significantly higher than that in LTBI group （ U = 1. 000, P = 0. 004 3）. ELISA analysis revealed there was no difference in NAP3 concentration in PBMCs culture supernatants before stimulation among the three groups （ F =0. 7341, P=0. 482 1）, but there was significant difference in NAP3 concentration after stimulation between active tuberculosis group and non-tuberculous infection healthy control group （ P =0. 002）. Conclusions High expression of NAP3 can be used as a biomarker for diagnosis of tuberculosis, but latent infection and active infection can not be distinguished.

关 键 词：结核 趋化因子 中性粒细胞激活蛋白3 结核潜伏感染 

分 类 号：R52[医药卫生—内科学]