蚕蛹虫草中虫草素和腺苷含量影响因素研究  

Study on Influence Factors of Cordycepin and Adenosine Contents in Cordyceps militaris

作　　者：施新琴[1] 顾寅钰[1] 郭光[1] 朱红[1] 李化秀[1] 赵德安[1] 王玺兰 

机构地区：[1]山东省蚕业研究所,山东烟台264002

出　　处：《山东农业科学》2015年第1期109-111,共3页Shandong Agricultural Sciences

基　　金：国家蚕桑产业技术体系烟台试验站;山东省农业重大应用技术创新课题(2013);山东省现代农业产业技术体系食用菌产业创新团队烟台综合试验站;山东省农业科学院青年基金(2007YQN023);山东省现代农业产业技术体系建设项目(NO.SDAIT-11-011-14;NO.SDAIT-17-011-06)

摘　　要：以双蒸水为溶剂,用反相高效液相色谱-紫外检测法测定蚕蛹虫草样品中虫草素和腺苷含量,并用该方法测定了19-3、17-3、MS、1-1以及1-Y等12种不同虫草菌株栽培的蚕蛹虫草、不同品质的蚕蛹虫草以及蚕蛹虫草不同组织中的虫草素和腺苷的含量。结果表明:1-Y菌株的虫草素和腺苷含量均最高,质量分数达15.45 mg/g和4.40 mg/g;不同品质的蚕蛹虫草中虫草素则以感染而未出草的僵蚕最高;蚕蛹虫草的僵蚕体对虫草素的富集能力高于子座,蚕蛹虫草的子座对腺苷的富集能力高于僵蚕体。该结果可对探求高虫草素含量和高腺苷含量的虫草材料提供理论依据。The contents of cordycepin and adenosine in Cordyceps militaris samples of different qualities,different parts and inoculated with 12 different strains such as 19- 3,17- 3,MS,1- 1 and 1- Y were determined by RP- HPLC using double distilled water as solvent. The results indicated that the contents of cordycepin and adenosine in 1- Y were both the highest,and the mass fraction reached 15. 45 mg / g and 4. 40 mg / g respectively. In Cordyceps militaris of different qualities,the content of cordycepin in silkworm infected but no fruiting were the highest. The enrichment ability of silkworm body to cordycepin was higher than stroma,while that of stroma to adenosine was higher than silkworm body. These results provided theoretical basis for seeking Cordyceps militaris with high cordycepin and adenosine contents.

关 键 词：虫草素 腺苷 含量 影响因素 高效液相色谱 

分 类 号：S886.9[农业科学—特种经济动物饲养]