17β-雌二醇对绵羊输卵管上皮细胞β-防御素-2(SBD-2)表达的影响  

Effects of 17β-estradiol on β-defensin-2(SBD-2) Expression in the Ovine Oviduct Epithelial Cells

作　　者：包图雅[1] 纳仁高娃[1] 白萨日娜[1] 李雅静[1] 杨燕燕[2] 

机构地区：[1]内蒙古医科大学基础医学院,内蒙古呼和浩特010110 [2]内蒙古农牧业科学院,内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2015年第1期10-13,共4页Animal Husbandry and Feed Science

基　　金：内蒙古自然科学基金项目(2014BS0801);内蒙古医科大学博士启动基金(NY2011BQ003);内蒙古医科大学青年创业基金(NY2010QN003)

摘　　要：[目的]探讨17β-雌二醇(E2)对体外培养的绵羊输卵管上皮细胞内抗菌肽SBD-2基因表达的影响。[方法]根据E2添加剂量设10-6mol/L组、10-7mol/L组、10-8mol/L组、10-9mol/L组和10-10mol/L组,各组细胞于加药后2、6、12、24及48 h,分别采用real-time RT-PCR技术检测E2对绵羊输卵管上皮细胞内SBD-2 m RNA表达量的影响,同时设相应的对照组。[结果]以剂量为10-8mol/L的E2处理输卵管上皮细胞6 h后,其SBD-2的表达量达到极值,极显著高于对照组(P<0.01),以剂量为10-10mol/L的E2处理输卵管上皮细胞24 h和48 h后,也能显著促进SBD-2的表达(P<0.01,P<0.05),之后随着各时间段E2剂量的增加,SBD-2 m RNA的表达量呈逐渐降低趋势。[结论]一定剂量的E2能够在处理细胞后的特定时间促进绵羊输卵管上皮细胞中SBD-2 m RNA的表达,E2对输卵管上皮细胞SBD-2 m RNA表达的调节作用存在时间效应与剂量依赖关系。[ Objective ]To investigate the effect of 17β-estradiol （E2） on mRNA expression levels of the [^-defensin-2 （SBD-2） in ovine oviduct epithelial ceils in vitro. [Method ]The primary ovine oviduct epithelial cells were isolated and subeuhured to 2nd passage. The 2nd passage cells were divided into five groups and were treated with 10^-6 tool/L, 10^-7 mol/L, 10^-8mol/L, 10-9 mol/L and 10^-10 mol/L of E2, respectively. The mRNA expression levels of the β-defensin-2 （SBD-2） of ovine oviduct epithelial cells were determined at 2^th h, 6^th h, 12^th, 24^th and 48^th after they were treated with different concentration of E2 by real-time RT- PCR, respectively. The blank control groups were also included in each E2 treated groups for each detection time. [ Results ] The mRNA expression of SBD-2 of ovine oviduct epithelial cells reached the extreme level when they were treated with E2 of 104 mol/L for 6 h and was significantly upregulated compared with the control（P〈0.01 ）, and the mRNA expression of the SBD-2 of the cells were also upregulated when they were treated with E2 of 10^-10 mol/L for 24 h and 48 h. The expression of the SBD-2 of the cells showed a gradual decrement trend with the increase of E2 concentration. [ Conclusion 3 It was indicated that the mRNA expression of SBD-2 of ovine oviduct epithelial cells can be upregnlated by specific concentration of E2 for certain time, and the effect of E2 on the expression of the SBD-2 of ovine oviduct epithelial cells showed a time effect and dose-dependent manner.

关 键 词：17Β-雌二醇 绵羊 输卵管上皮细胞 SBD-2 MRNA表达量 

分 类 号：Q492.5[生物学—生理学]