无患子皂苷诱导人肺癌A549细胞凋亡的作用及其机制研究  

Experimental Study on Apoptosis Induced by Sapindus Saponin in Human Pulmonary Carcinoma Cell A549 and Its Molecular Mechanisms

作　　者：王美玲[1] 胡小红[1] 

机构地区：[1]广州中医药大学中药资源科学与工程研究中心/岭南中药资源教育部重点实验室,广东广州510006

出　　处：《中医药导报》2015年第4期35-38,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中央财政支持地方高校发展专项资金项目(粤财教[2010]358号)

摘　　要：目的:探讨无患子皂苷对人肺癌A549细胞的影响及其可能的分子机制。方法:采用MTT法检测无患子皂苷溶液对A549细胞增殖抑制作用;Hoechst 33258荧光染色和流式细胞术观察和检测细胞凋亡情况;实时荧光定量PCR法检测凋亡基因bcl-2 m RNA的表达。结果:无患子皂苷能显著抑制A549细胞的增殖,且呈浓度依赖性;Hoechst 33258荧光染色和流式细胞术结果显示无患子皂苷可以诱导A549细胞凋亡,实时荧光定量PCR法表明不同浓度的无患子皂苷均能明显降低bcl-2的表达。结论:无患子皂苷可通过诱导细胞凋亡而抑制A549细胞增殖,其作用可能与下调细胞凋亡相关基因bcl-2的表达有关。Objective： To investigate the apoptosis induced by Sapindus Saponin（SPSP） in human lung cancer cell A549 and its molecular mechanisms. Methods： Using MTT assay, the inhibition of SPSP on the proliferation of A549 cells was detected;changes of the cell morphology and effects on apoptosis were observed by Hoechst 33258 and flow cytometry; real time PCR was used to detect m RNA expressions of bcl-2. Results： SPSP significantly inhibited the growth of A549 cells in a dose-de-pendent manner. The test of Hoechst 33258 and flow cytometry indicated that SPSP could induce the apoptosis of A549 cells.Real time PCR indicated that SPSP could obviously decrease the expression of bcl-2. Conclusion： SPSP could promote the apoptosis of A549 cells, which may be related to the decrease of bcl-2 expression.

关 键 词：无患子皂苷 肺癌A549细胞 细胞凋亡 

分 类 号：R273[医药卫生—中西医结合]