MIF对低氧诱导肺血管成纤维细胞增殖和分化的影响  

Effect of Macrophage Migration Inhibitory Factor on Proliferation and Differentiation of Pulmonary Vascular Fibroblasts Cells Induced by Hypoxia

作　　者：杨威[1] 张小强[1] 董啸[1] 周建良[1] 吴永兵[1] 龚艺[1] 徐建军[1] 

机构地区：[1]南昌大学第二附属医院心胸外科,江西南昌330008

出　　处：《临床医学工程》2014年第12期1525-1526,共2页Clinical Medicine & Engineering

基　　金：国家自然科学基金项目"前B细胞克隆增强因子在体外循环术后肺血管内皮通透性增加中的机制研究"(项目编号:81260054);国家自然科学基金项目"VEGF165/OPG共价修饰去细胞瓣重构介入心脏瓣膜的抗钙化机制研究"(项目编号:81260047);江西省教育厅科学技术研究项目(项目编号:GJJ10320)

摘　　要：目的探讨巨噬细胞移动抑制因子(MIF)在低氧诱导肺血管成纤维细胞增殖和转分化的影响。方法 1%氧浓度诱导肺动脉成纤维细胞低氧细胞,并被分为6组,分别被处以培养基、MIF特异性阻断剂ISO-1(35 mg·kg-1·d-1)、二甲基亚砜(DMSO)、抗MIF抗体、抗CD-44抗体和非特异性Ig G。Western印迹法检测每孔细胞裂解液的α-SMA的表达水平;流式细胞仪检测溴脱氧尿苷(Brd U)和α-SMA阳性细胞数;采用LDH试剂盒,严格按照说明书检测每组细胞LDH含量。结果加入MIF特异性阻断剂ISO-1和抗MIF抗体的小组α-SMA水平最低(P<0.05),Brd U和α-SMA阳性细胞数目最低(P<0.05)。LDH活性在各组无显著性差异(P>0.05)。结论成纤维细胞低氧诱导MIF在早期表达,MIF与成纤维细胞增殖和分化相关。Objective To investigate the effect ofmacrophage migration inhibitory factor （MIF） on proliferation and differemiation of pulmonary vascular fibroblasts cells induced by hypoxia. Methods Pulmonary vascular fibroblasts cells were incubated in 1% oxygen to present the hypoxia-inducible cells and divided into six groups, given with culture medium, ISO-1 （35 mg·kg-1·d-1）, DMSO, anti-MIF antibody, anti-CD-44 antibody and non-specific IgG respectively. The levels of a-SMA were detected by western blotting, the number of BrdU and ot-SMA positive cells were detected by flow cytometry, the activities of LDH were detected by LDH cytotoxicity kit. Results The levels of α-SMA, the number of BrdU and α-SMA positive cells in the group adding ISO-1 （35 mg·kg-1· d-1） and anti-MIF antibody were the lowest （all P 〈0.05）. The LDH activity in these groups had no significant difference （P 〉0.05）. Conclusions Pulmonary vascular fibroblasts cells induced by hypoxia express MIF at early phase and MIF correlates with its proliferation and differentiation.

关 键 词：巨噬细胞移动抑制因子 肺成纤维细胞 增殖和分化 

分 类 号：R543.2[医药卫生—心血管疾病]