Gene Manipulation Based Selenium-containing Peptide Exhibiting Synergism of SOD and GPx  

作　　者：SHEN Na YAN Fei LU Shaowu YANG Wenkui LUO Guimin YAN Ganglin MU Ying 

机构地区：[1]Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130021, P. R. China [2]Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control,State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, P. R. China

出　　处：《Chemical Research in Chinese Universities》2014年第6期947-952,共6页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（Nos.31170740, 31270907） and the Natural Science Founda- tion of Jilin Province, China（No.20130101159JC）.

摘　　要：In this paper, we constructed a novel bifunctional superoxide dismutase（SOD）/glutathione peroxi- dase（GPx） mimic, a selenium-, copper-containing 35-mer peptide conjugate（Se-Cu-35P） in which a three-amino acid linker（（31y-Asn-Gly） connects the C-terminus of 17-mer polypeptide SOD mimic with the N-terminus of 15-mer po- lypeptide GPx mimic. The SOD and GPx activities of Se-Cu-35P are two orders of magnitude lower than those of natural SOD and GPx, respectively. It provides a GPx activity 56-fold higher than Ebselen（a well-known GPx mimic）. The glutathione（GSH） binding constant is 5.6× 10^2 L.mol 1. Se-Cu-35P synergistically resists against the inactivation by H202 and protects the mitochondria from oxidative damage in a dose dependent manner. These results highlight the challenge of generating an efficient SOD/GPx synergism mimic. It could facilitate the studies of the cooperation of GPx and SOD and could be a potential therapeutic agent for the treatment of ROS-mediated diseases,In this paper, we constructed a novel bifunctional superoxide dismutase（SOD）/glutathione peroxi- dase（GPx） mimic, a selenium-, copper-containing 35-mer peptide conjugate（Se-Cu-35P） in which a three-amino acid linker（（31y-Asn-Gly） connects the C-terminus of 17-mer polypeptide SOD mimic with the N-terminus of 15-mer po- lypeptide GPx mimic. The SOD and GPx activities of Se-Cu-35P are two orders of magnitude lower than those of natural SOD and GPx, respectively. It provides a GPx activity 56-fold higher than Ebselen（a well-known GPx mimic）. The glutathione（GSH） binding constant is 5.6× 10^2 L.mol 1. Se-Cu-35P synergistically resists against the inactivation by H202 and protects the mitochondria from oxidative damage in a dose dependent manner. These results highlight the challenge of generating an efficient SOD/GPx synergism mimic. It could facilitate the studies of the cooperation of GPx and SOD and could be a potential therapeutic agent for the treatment of ROS-mediated diseases,

关 键 词：Reactive oxygen species Superoxide dismutase Glutathione peroxidase Bifunctional enzyme 

分 类 号：Q554.9[生物学—生物化学] S858.31[农业科学—临床兽医学]