陕西设施蔬菜根结线虫特异性分子检测  

Specific Molecular Assay for the Detection of Greenhouse Vegetables Meloidogyne spp. in Shaanxi

作　　者：张锋[1] 杨苗苗[2] 孙娟[3] 洪波[1] 张淑莲[1] 

机构地区：[1]陕西省动物研究所,西安710032 [2]陕西学前师范学院,西安710100 [3]富平县农技中心,陕西富平711700

出　　处：《中国农学通报》2014年第31期136-140,共5页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划"丘陵沟壑区高效农业生产关键技术集成与示范"(2011BAD31B05-04);陕西省科学院重大科技项目"大荔现代特色农业产业保障技术研究与示范"(2014K-03)

摘　　要：为明确陕西设施蔬菜根结线虫的主要种类,利用已报道的花生根结线虫[Meloidogyne arenaria(Neal.)Chitwood]、南方根结线虫[Meloidogyne incognita(Kofold&White)Chitwood]、北方根结线虫(Meloidogyne hapla Chitwood)和爪哇根结线虫[Meloidogyne javanica(Treub)]的特异性引物,对陕西省20个县市区的24个样区的根结线虫进行PCR检测。样品中检测到了南方根结线虫、北方根结线虫、花生根结线虫和爪哇根结线虫,其中南方根结线虫为优势种群,大部分样区为单一种群为害,但个别样区存在2种种群。In order to identify the species of vegetable root-knot nematodes in Shaanxi Province,the reported specific primers of Meloidogyne arenaria（Neal.） Chitwood, Meloidogyne incognita（Kofold White） Chitwood,Meloidogyne hapla Chitwood and Meloidogyne javanica（Treub） were served as polymerase chain reaction （PCR） assay primers respectively to detect Meloidogyne spp. of greenhouse vegetables that obtained from twenty four sampling areas that distributed in twenty cities and counties in Shaanxi. Our study showed that M.arenaria, M. incognita, M. hapla and M. javanica were detected, and M. incognita was the dominant species;most sampling areas were infected by single population, however, specific sampling areas were infected by two populations.

关 键 词：根结线虫 PCR检测 特异性引物 分子鉴定 

分 类 号：S641.2[农业科学—蔬菜学] S432.45[农业科学—园艺学]