接种摩西柄囊霉对连作大豆苗期根腐病病原真菌多样性的影响  

Funneliformis mosseas on Soybean Seedling Root Rot Fungi Diversity of Continuous Cropping

作　　者：范雪露[1,2] 崔佳琦 蔡柏岩[1,2,3] 

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080 [2]黑龙江省普通高等学校微生物学重点实验室,哈尔滨150080 [3]黑龙江东方学院,哈尔滨150086

出　　处：《中国农学通报》2014年第31期272-279,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金面上资助项目"连作大豆土壤AM真菌菌群结构分析及其与根腐病病原菌相互关系研究"(31170466);黑龙江省教育厅面上资助项目"连作大豆土壤AM真菌菌群结构分析及其与根腐病病原菌相互关系研究"(12521394);黑龙江大学高层次人才支持计划项目"生态修复团队"(Hdtd2010-12)

摘　　要：通过对不同连作年限的3个大豆品种接种F.mosseas,分析大豆根腐病高发期苗期根系致病真菌的多样性,旨在证明接种F.mosseas能否抑制根腐病病原真菌对大豆根系的侵染,减轻大豆根腐病的发生。采用PCR-DGGE技术分析不同连作年限大豆苗期根系根腐病病原真菌的多样性变化,同时对DGGE图谱进行丰度、优势度、多样性分析。结果表明:接种F.mosseas对大豆根系根腐病病原真菌的多样性具有显著的影响,多样性批数明显下降,说明接种F.mosseas对连作大豆根腐病致病真菌的生长具有抑制作用。因此,为采用AM菌剂预防连作大豆根腐病的发生进行了有益的探索,对解决连作大豆根腐病发生具有重要的实践意义。In this study, the author inoculated F.mosseas to the three soybean varieties of different continuous cropping years to analyze the diversity of fungi during the high-incidence of seedling root rot. In order to inhibit the fungi infect the root of soybean and to reduce the incidence of root rot. Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis（PCR- DGGE） was used to analyze the diversity of fungi in the seedling of continuous cropping and the abundance, dominance and index of diversity from DGGE profiles. The results showed that： inoculated F.mosseas had a significant effect on the diversity of fungi and decreased the index of diversity significantly. The study stated that F.mosseas could inhibit the growth of the root rot fungi in the continuous cropping. Therefore, the study has a practical significance on exploring AMF prevention of the incidence of root rot fungi in the continuous cropping.

关 键 词：大豆 F.mosseas 根腐病病原真菌 PCR-DGGE 

分 类 号：S181[农业科学—农业基础科学]