甲草胺在玉米籽粒上的QuEChERS-HPLC残留分析方法探究  

QuEChERS-HPLC Residue Analysis Method of Alachlor in Corn

作　　者：汪玉[1] 高子登 张剑南[1] 吕连启[2] 刘冬梅[2] 胡树文[1] 

机构地区：[1]中国农业大学资源与环境学院环境科学与工程系,北京100193 [2]济宁市任城区农业局,山东济宁272100

出　　处：《中国农学通报》2014年第31期295-299,共5页Chinese Agricultural Science Bulletin

基　　金：农业部公益性行业专项"一次性施肥技术及推广示范"(201303103);科技部"十二五"科技支撑计划"环境友好型高分子膜材料研发"(2011BAD11B02)

摘　　要：建立了一种简单、快速、环保的甲草胺在玉米籽粒中的QuEChERS-HPLC残留分析方法。样品用极少量的乙腈提取,用N-丙基乙二胺（PSA）和石墨化炭黑（GCB）净化,高效液相色谱（紫外检测器）检测甲草胺浓度。色谱柱为C18反相柱（250 mm×4.6 mm×5.0μm）,流动相乙腈和水（70：30）溶液。对玉米中的甲草胺进行不同水平的添加回收率实验,在考察浓度范围内线性关系良好（R^2≥0.994）;在0.2~10 mg/kg添加水平内,平均加标回收率为96.7%~113.8%,相对标准偏差（RSD）为3.2%~15.3%;检出限和定量限分别为0.015mg/kg、0.05mg/kg。该方法的精密度、灵敏度和准确度符合农药残留分析的要求。A simple, fast, and environmental-friendly determination method of alachlor residue in corn was established using QuEChERS- HPLC equipped with UV detector. Samples were extracted by a little acetonitrile, purified by N- propyl ethylenediamine（PSA） and graphitized carbon black（GCB）. The concentration of alachlor was detected by HPLC-UV. The analytical column was C18（250 mm×4.6 mm×5.0 μm）and the mobile phase was acetonitrile mixed with water（70：30 V/V）. With a significant linear relation （R^2≥0.994） of standard samples measured by this method, the average recovery rates of different alachlor doses（0.2-10 mg/kg） were 96.7%-113.8% and RSD were 3.2%-15.3%. The limit of detection and the limit of quantitation were 0.015 mg/kg and 0.05 mg/kg respectively. The accuracy, sensitivity and precision of this method can meet the requirement of pesticide residue analysis.

关 键 词：QUECHERS 甲草胺 玉米 高效液相色谱 

分 类 号：X830.2[环境科学与工程—环境工程]