市售草鱼鳃中细菌的分离鉴定及耐药性分析  

Identification and Antimicrobial Resistance Analysis of Bacteria Isolated from the Gills of Commercial Grass Carp (Ctenopharyngodon idellus)

作　　者：胡秀彩[1] 兰云[2] 沈晓静[2] 吕爱军[1] 朱爱华[1] 

机构地区：[1]江苏师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116 [2]江苏师范大学生命科学学院,江苏徐州221116

出　　处：《中国农学通报》2014年第32期43-47,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"嗜水气单胞菌诱导斑马鱼皮肤免疫应答的分子机制"(30800847);江苏省"青蓝工程"(2014)

摘　　要：对徐州市售草鱼细菌带染状况及耐药性分析,结果表明从其鳃中分离到4株革兰氏阴性短杆菌,暂时编号为ZYF-1、ZYF-2、ZYF-3和ZYF-4,查阅数值编码鉴定ZYF-1菌株为志贺菌(Shigella sp.),ZYF-2、ZYF-3菌株为大肠埃希菌(Escherichia coli),ZYF-4菌株为泉居沙雷菌(Serratia fonticola)。药敏试验结果表明,4株分离菌的耐药率分别为75.8%、72.4%、65.5%和82.8%,均对氨苄西林、阿莫西林、复方新诺明、磺胺异恶唑和甲氧苄啶等11种抗生素耐药。本研究显示市售草鱼存在志贺菌、大肠埃希菌带染现象,对多种抗生素耐药,这为食源性细菌鉴定及鱼病防治提供参考。Investigation was conducted on bacterial contamination and antimicrobial resistance of the strains isolated from the gills of commercial Grass carp（Ctenopharyngodon idellus） in Xuzhou, four isolated strains were gram-negative and short rod-shaped bacteria, and tentatively named ZYF-1, ZYF-2, ZYF-3 and ZYF-4, respectively. According to the values of coding method of four strains, ZYF-1 was identified as Shigella sp.,ZYF-2 and ZYF-3 were identified as Escherichia coli, and ZYF-4 was identified as Serratia fonticola. The antimicrobial susceptibility assay indicated that the resistance rates of ZYF-1, ZYF-2, ZYF-3 and ZYF-4 strains were 75.8%、72.4%、65.5% and 82.8%, respectively. Four strains showed antibiotic resistance to eleven kinds of antibiotics, such as ampicillin, amoxicillin, trimethoprim- sulfamethoxazole, sulfisoxazole,trimethoprim and so on. It is concluded that there are Shigella and Escherichia coli contamination in commercially available Ctenopharyngodon idellus in Xuzhou, and the bacteria showed multi-drug resistances in antimicrobial susceptibility assay. The conclusion will provide scientific references for identification of foodborne bacteria and control of bacterial disease in fish.

关 键 词：草鱼 鳃 分离鉴定 药敏试验 

分 类 号：R378[医药卫生—病原生物学]